TF Amber_价格-厂家-供应商_郑州麦斯凯生物技术开发有限公司_丁香通

TFAmber
Reference 499558-250
Quantity 250 nmol
Store at – 20 °C

Properties of TFAmber when bound to FAST2
Excitation wavelength 499 nm
Emission wavelength 558 nm
Molar absorption coefficient 46,000 M–1 cm–1
Fluorescence quantum yield 57 %
Affinity constant at 25° C 0.06 µM

TFAmber is a membrane-permeant fluorogenic ligand that can be used to selectively label FASTtagged proteins in solution, in living cells and in fixed cells. TFAmber is almost non-fluorescent when free in solution, but strongly fluoresces when bound to FAST1 or FAST2 or their tandem versions tdFAST1 and tdFAST2 (for its properties with FAST1 please visit our website). This package includes 250 nmol of TFAmber, enabling to prepare 50 mL of a 5 µM labeling solution.

The Twinkle Factory labeling technology is a novel tool that enables the specific fluorescent labeling of any protein of interest. This technology is based on the instantaneous formation of a fluorescent molecular assembly between the small (14 kDa) protein tag FAST and various fluorogenic ligands ( TFFluorogens). TFFluorogens strongly fluoresce only when bound to FAST, enabling to detect and image FAST-tagged proteins with high contrast without the need of washing the excess of fluorogenic ligands. The labeling of FAST-tagged proteins with a TFFluorogen is non-covalent and can be reversed if necessary by washing. By using different TFFluorogens, the spectral properties of the FAST-tagged protein can be changed without the need to switch protein tags, providing an experimental versatility not encountered with fluorescent proteins.

The use of the Twinkle Factory labeling technology implies cloning and expression of the FASTtagged protein, and labeling of the resulting fusion with the TFFluorogen of choice. The labeling of FAST-tagged proteins is described below. Cells expressing FAST-tagged proteins are not supplied. Note that proteins of interest can be expressed with FAST as either an N- or a C-terminal fusion.
 
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