Global urinary metabolic profiling procedures using gas chromatography–mass spectrometry

Globalurinarymetabolicprofilingproceduresusinggaschromatography–massspectrometryEricChunYongChan,1KishoreKumarPasikanti1,2&JeremyKNicholson3

Journalname:NatureProtocolsVolume:6,Pages:1483–1499Yearpublished:(2011)DOI:doi:10.1038/nprot.2011.375Publishedonline08September2011

Abstract

TheroleofurinarymetabolicprofilinginsystemsBIOLOGyresearchisexpanding.ThisisbecauseoftheuseofthistechnologyforclinicaldiagnosticandmechaNISTicstudiesandforthedevelopmentofnewpersonalizedhealthcareandmolecularepidemiology(population)studies.ThemethodologiescommonlyusedformetabolicprofilingareNMRspectroscopy,liquidchromatographymassspectrometry(LC/MS)andgaschromatography–massspectrometry(GC/MS).Inthisprotocol,wedescribeurinecollectionandstorage,GC/MSanddatapreprocessingmethods,chemometricdataanalysisandurinaryMarkermetaboliteidentification.ResultsobtainedusingGC/MSarecomplementarytoNMRandLC/MS.SamplepreparationforGC/MSanalysisinvolvesthedepletionofureaviatreatmentwithurease,proteinprecipitationwithmethanol,andtrimethylsilylderivatization.Theprotocoldescribedherefacilitatesthemetabolicprofilingof~400–600metabolitesin120urinesamplesperweek.

原文：http://www.nature.com/nprot/journal/v6/n10/abs/nprot.2011.375.html