一抗 | 特异性一抗 | 赛默飞世尔官网 | Thermo Fisher Scientific CN

一抗是一种免疫球蛋白，能特异性结合特定蛋白或重点研究的其他生物分子，目的是对其进行纯化、检测和测量。使用小鼠、大鼠、兔子、山羊和其他动物作为寄主，可将一抗培育成多克隆或单克隆抗体。一抗的生产和供应有多种形式，从抗血清粗品到抗原纯化制剂不等。常用于研究目标的一抗还提供与荧光染料或生物素结合的产品形式。

领取新人买即送2重礼

We are continuously updating our Invitrogen primary antibody portfolio with advanced verified products for use in flow cytometry, IHC/IF/ICC, western blotting, ELISA, and other applications. Checkout our latest antibody highlights:

Rabbit recombinant monoclonal MAVS antibody for immuno-oncology research

MAVS (Mitochondrial antiviral-signaling protein), also known as virus induced signaling adaptor (VISA), IFN-β promoter stimulator I (IPS-1), or caspase activation recruitment domain adaptor inducing IFN-β (CARDIF), is a protein present in the outer membrane of mitochondria and peroxisomes. This protein plays a key role in innate immunity to viral infections. MAVS functions in RNA-sensing pathways, integrating signals from activated sensors, like RIG-1 and MDA5, upon viral infection and ultimately leading to proinflammatory cytokine production and programmed cell death of the infected cells. Recently, these pathways have been identified as targets for immune therapy in cancer, as it has been shown that activation of these pathways can directly induce programmed cell death in the cancer microenvironment.

Currently, few MAVS antibodies have been tested for their specificity. However, the recently launched Invitrogen MAVS Recombinant Rabbit Monoclonal Antibody (Cat. No. 703153) is CRISPR-verified to ensure specificity and has been tested in western blotting.

MAVS rabbit recombinant monoclonal antibody shows human reactivity and is knock-out verified. (A) Western blot analysis was performed using membrane-enriched extracts (30 µg lysate) of HeLa (lane 1), THP-1 (lane 2), A549 (lane 3), Hep G2 (lane 4), and NIH/3T3 (lane 5) cells. A ~60 kDa band corresponding to MAVS was observed only in the human cell lines tested. In Hep G2 cells, the MAVS antibody also recognizes a smaller isoform, evident by a smaller band at ~55 kDa. Detection was performed using the Invitrogen ECL Chemiluminescent Substrate Reagent Kit (Cat. No. WP20005). (B) Antibody specificity was demonstrated by CRISPR-Cas9-mediated knockout (KO) of the target protein in 293T cells. A loss of signal was observed for the target protein in a MAVS KO cell line compared to the Cas9 control cell line.

该视频演示了一抗的双标记。观看如何创造荧光抗体-药物偶联物，并了解其在蛋白质检测基本功能以外的应用。

Invitrogen抗体介绍

Invitrogen™提供超83,000种经严格验证的一抗二抗，反应种属超60种，覆盖蛋白质组中超过91%的靶点蛋白

Invitrogen抗体覆盖50个研究领域，包括：癌症、干细胞、神经生物学、免疫学、表观遗传学

Invitrogen抗体支持9大应用领域，包括：

实用工具 2分钟搞定抗体选择：Invitrogen抗体选择工具
Invitrogen抗体质量保障承诺

轻松选，放心购

只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符，请联系我们的技术服务团队。如经过技术认证，由于质量问题而无法获得预期结果，您即可享受免费退换货服务。

了解详情 抗体质量保障承诺
搜索特异性一抗

使用以下搜索工具查找感兴趣的抗体。然后，按照靶标或宿主物种、单克隆或多克隆抗体类型以及其他标准过滤结果。

研究领域一抗

查找和挑选适合于关键标记和目标（与癌症研究、干细胞研究、神经生物研究、表观遗传研究和更多研究相关）的一抗。

信号通路一抗

查看对关键信号通路、转录通路和各种不同的磷酸化或活化状态蛋白质特异的一抗菜单和列表。

表位标记一抗等

查找对荧光蛋白、荧光染料、融合蛋白和报告蛋白、生物素以及抗原表位标记（例如 GST、His、HA 和 Myc）特异的一抗信息。

细胞标记一抗

选择细胞标记、细胞器标记或亚细胞组分一抗，通过检测对其特异的蛋白，鉴别和显示细胞结构和细胞器。

流式细胞术抗体

Invitrogen eBioscience Super Bright 聚合物染料将加入 Alexa Fluor 染料与 eFluor 染料抗体偶联物产品组合，帮助您解答复杂的细胞生物学问题，用时更短，更加节约样本。

偶联一抗

查找和挑选已直接标记或与生物素亲和标记物、荧光染料（Invitrogen™ Alexa Fluor™ 染料）或报告酶 (HRP, AP) 偶联的一抗。

重组兔单克隆抗体

了解我们的 Invitrogen ABfinity™ 重组兔单克隆抗体，其培育方法为：对动物进行免疫、功能筛查、然后将基因克隆到高级哺乳类动物表达载体。

Cell Marker Antibodies

Select a cell marker, organelle marker, or subcellular component antibody to identify and outline cellular structures and organelles by detecting proteins that are specific to them.

对照抗体

查找加载型和同型对照抗体，适用于对半定量印迹和其他免疫测定结果进行标定、标准化和验证。

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