Mouse Spleenectomy

Advanced BioMatrix代理 Advanced BioMatrix- PureCol Advanced BioMatrix- GelCol Advanced BioMatrix- Nutragen Advanced BioMatrix- FibriCol Advanced BioMatrix- TeloCol Advanced BioMatrix- VitroCol 详情介绍 PureCol® Type I Collagen Solution, 3 mg/ml (Bovine)产品介绍品牌：advancedbiomatrix货号：5005规格：100ml，1000ml美金：100ml 380 1000ml 3350PureCol是一种3mg/ml的I型牛非胶原蛋白溶液，用于二维和三维细胞培养，或作为标准胶原蛋白。PureCol被称为胶原蛋白产品纯度（99.9%胶原蛋白含量）、一致性和重现性的标准，并被2000多篇出版物引用。产品描述：PureCol®胶原蛋白被认为是所有胶原蛋白纯度（ 99.9%胶原蛋白含量）、功能性和天然的胶原蛋白的标准。PureCol®是从来自美国wei yi 受控制的封闭牛群的牛皮中分离出来的。的BioMatrix生产工艺符合严格的质量标准，证明其具有的批量一致性。PuReCl®胶原蛋白是大约97%型I型胶原胶原，其余的由III型胶原组成。PuReCl®胶原蛋白以大约3毫克/毫升的浓度供应。每批产品的浓度都在购买每种产品时提供的分析证书上。PureCol®是可溶于0.01 N HCI中的非胶原蛋白，因此，pH值为2。PureCol®胶原蛋白是表面涂层、为培养细胞提供薄层制备或用作固体凝胶的理想材料。PureCol®胶原蛋白以方便用户使用和储存的包装提供。PureCol®经过无菌过滤，作为即用溶液提供。Product NumberProduct NameBrandPrice5005-100MLPureCol®, Bovine Collagen, 3 mg/mLAdvanced BioMatrix$365.005074-35MLPureCol® EZ Gel, Bovine Collagen, DMEM/F-12 SolutionAdvanced BioMatrix$333.005006-15MGPureCol®, Bovine Collagen, Lyophilized, 15 mgAdvanced BioMatrix$208.005167-100MLGelCol™ Bovine Collagen Solution, Type I, 3 mg/ml, 100 mlAdvanced BioMatrix$315.00010-50MLNutragen®, Bovine Collagen, Solution, 6 mg/mLAdvanced BioMatrix$395.005133-20MLFibriCol®, Bovine Collagen Solution, 10 mg/mLAdvanced BioMatrix$317.005007-20MLVitroCol®, Human Collagen, Solution, 3 mg/mLAdvanced BioMatrix$296.005008-15MGVitroCol®, Human Collagen, Lyophilized, 15 mg CatalogAdvanced BioMatrix$262.005026-1KITTeloCol®-3, Solution, Acid Soluble, 3 mg/ml #5026-1KITAdvanced BioMatrix$260.005225-1KITTeloCol®-6, Solution, Acid Soluble, 6 mg/ml #5225-1KITAdvanced BioMatrix$395.005226-20MLTeloCol®-10, Solution, Acid Soluble, 10 mg/mlAdvanced BioMatrix$290.005169-100MLFlexiCol™ Porcine Collagen Solution, Type I, 3 mg/ml, 100 mlAdvanced BioMatrix$406.005153-1KITRat Tail Collagen Solution, Acid Soluble with Neutralization Solution, 100 mgAdvanced BioMatrix$150.005056-20MLRat Tail Collagen Solution, Acid Soluble, 4 mg/mlAdvanced BioMatrix$150.005162-1GMBovine Collagen, Type I, Lyophilized Fibrous Powder, 1 gramAdvanced BioMatrix$135.005164-5GMBovine Collagen, Type I, Lyophilized Fibrous Sheet, 5 grams CatalogAdvanced BioMatrix$315.005202-1KITHigh Concentration Collagen, 35 mg/mlAdvanced BioMatrix$230.005021-10MGHuman Collagen Solution, Type III, 10 mgAdvanced BioMatrix$191.005019-10MGHuman Collagen Powder, Type III, Non-sterile, 10 mgAdvanced BioMatrix$146.005086-0.1MGCD2, Human, Recombinant, 0.1 mgAdvanced BioMatrix$213.005124-0.05MGCDH3, Human, Recombinant, 0.05 mgAdvanced BioMatrix$172.005125-0.1MGCDH13, Human, Recombinant, 0.1 mgAdvanced BioMatrix$213.005089-0.1MGCD14, Human, Recombinant, 0.1 mgAdvanced BioMatrix$213.005090-0.1MGCDH18, Human, Recombinant, 0.1 mgAdvanced BioMatrix$213.005093-0.1MGCD40, Human, Recombinant, 0.1 mgAdvanced BioMatrix$213.005096-0.1MGCD86, Human, Recombinant, 0.1 mgAdvanced BioMatrix$213.005100-0.05MGCD164, Human, Recombinant, 0.05 mgAdvanced BioMatrix$172.005127-0.1MGCD270, Human, Recombinant, 0.1 mgAdvanced BioMatrix$213.005126-0.1MGCD274, Human, Recombinant, 0.1 mgAdvanced BioMatrix$213.005123-0.1MGCD276, Human, Recombinant, 0.1 mgAdvanced BioMatrix$213.005085-0.1MGE-Cadherin, Human, Recombinant, 0.1 mgAdvanced BioMatrix$213.005107-0.1MGICAM2, Human, Recombinant, 0.1 mgAdvanced BioMatrix$213.005052-1MGTropoelastin, Human, Recombinant, Lyophilized, 1 mgAdvanced BioMatrix$333.00北京百奥创新科技有限公司：一、辐射五大服务方向： 1.国内试剂耗材经销代理； 2.国外试剂订购（直订，或境外代理）； 3.基因组测序（二代，二代+三代）； 4.基因分型（SNP检测）：低/中/中 高通量项目； 5.生物样本运输（国内、）；二、百奥创新多家授权代理品牌，涉及30多种，经营代理500多个品牌： Qiagen、Sigma、R D、Epigentek、ThermoFisher、DNA Genotek、USA Scientific、 Matreya、Sage Science、List Labs、Macrocyclics、Steraloids、 NIBSC、mobio、immunodx、cellsystems、medicago、abcam CST、Roche、Bethyl、sino Biological、clodrosome、adi BIO-WORLD、TOKU-E、HYPHEN BioMed、moltox、ZeptoMetrix International Humic、biocytex、Toxin Technology 、Streck Elastin、biomax、Orflo Technologies、Accegen、Advbiomart、genlantis、coaChrom cerilliant、Cygnus Technologies LLC、Enzyme Research、Bio/Data Corporation Greer Lab 、novusbio、Solarbio、bangslabs、bioline、musechem、Novocastra stemrd、hyphen-biomed、polyplus、moltox 、exiqon、pentapharm、verichemlabs GenStar、ALZET、lucernatechnologies 、meridian、origene、zimmerpeacocktech horizondiscovery、Reagents、megazyme、elisasystems 北京百奥创新科技有限公司所有生物试剂耗材都可以进口，特别是冷门品牌，均能给客户购买到。价格优，货期短，可加急，较快10天到货；保证服务质量，所有产品均提供售后服务。大部分客户我们提供货到付款服务， 百奥创新自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。客户包括北大，清华，地大，中科院各所，各大药企等等。 专业订购国内外试剂耗材，常备现货，一手货源，欢迎合作。 以上信息由企业自行提供，信息内容的真实性、准确性和合法性由相关企业负责，化工仪器网对此不承担任何保证责任。 温馨提示：为规避购买风险，建议您在购买产品前务必确认供应商资质及产品质量。

OUTLINEPROTOCOLGeneralanesthesia1.injecti.p.250µl/mouseoftheANESTHETICmixture<waitfor2-10minfortheanestheticeffect>2.forverifytheanestheticeffectcheckplantarreflexes(animalmustbeunresponsivetosqueezinghindfeet)Preparationofthesurgicalarea1.washthesurgicalandadjacentareawithC2H5OH-70%2.razethefurwiththerazor3.washthesurgicalareawithC2H5OH-70%Operation

1. Atthelefthypochondriummakea1.5-2cm"skin,musclesandperitoneum"incisionwithsurgicalscissors
2. Gentlytrapandpushthespleenfreeofadjacenttissue,separateitslegwithblunt-endedscissorsorforceps
3. Byasingleknottieoffthearterywitha3-0planeCATGUTbyloopingthesuturethroughthemesentary(thearteryentersthespleenatthesuperiorpolus).Ifnecessarybyasingleknottieofftheefferentvenule(exitsthespleenattheinferiorpolus)
4. Cutawaytheconnectivetissueandremovethespleen
5. Applyontheopenwound1pinchofAstrexene(ChlorhexidinHCl)(5mg/g)
6. ApplyontheperitoneumandmusclestheU-likesuturewithplaneCATGUT(3-0)
7. ApplyontheskintheU-likesuturewithETHICONMERSILENE(3-0)

Wakening1.inject250ul/mouseofthe"WAKENINGMIXTURE"Postoperationperiod

1. aftertheoperationtookplacelettheanimalstorestforatleast7daysinacagewiththefilter
2. observeanimalseveryday

SOLUTIONS1.ANESTHETICMIXTURE=mix1mlofDomitor(MedetomidinHCl)(1mg/ml),1mlofImalgene(KetaminumHCl)(50mg/ml)and8mlofPBS2."WAKENINGMIXTURE"=mix1mlofANTISEDAN(AtipamezoleHCl)(5mg/ml)and9mlofPBS