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BioAssay Systems/QuantiChrom™ Glutamate Dehydrogenase Assay Kit/100 tests/DGLDH-100

QuantiChrom™ Glutamate Dehydrogenase Assay Kit

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ProtocolSDS

Application

For quantitative determination of glutamate dehydrogenase activity.

Key Features

Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

OD565nm

Samples

Serum, plasma, cell, tissue, agriculture etc

Species

Size

100 tests

Detection Limit

0.1 U/L

Shelf Life

6 months

More Details

GLUTAMATE DEHYDROGENASE (GLDH) is an enzyme which catalyzes the interconversion of glutamate and a-ketoglutarate. Elevated blood serum GLDH levels indicate liver damage; thus, GLDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections. BioAssay Systems' non-radioactive, colorimetric GLDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

Ballester-Lozano, GF et al (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr.114(5):713-26. Assay: Glutamate dehydrogenase in sea bream liver cells. Laura BP et al. (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markersin gilthead sea bream (Sparus aurata). Aquaculture. 454 (2016) 8-18. Assay: Glutamate dehydrogenase in fish plasma. To find more recent publications, pleaseclick here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

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