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Biomol/Cas9-Expressing Neuro2A Cell Pool/2 vials/BPS-78087

Product information "Cas9-Expressing Neuro2A Cell Pool"
Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing Neuro2A cell pool can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
Keywords: SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1
Supplier: BPS Bioscience
Supplier-Nr: 78087

Properties

Application: Quick knock-out cell pool / cell line generation, SgRNA screening
Reactivity: Mouse

Database Information

KEGG ID : K09952 | Find alternatives
UniProt ID : Q99ZW2 | Find alternatives

Handling & Safety

Storage: Liquid nitrogen (store in liquid nitrogen)
Shipping: -80°C (International: °C)
Caution Our products are for laboratory research use only: Not for administration to humans!
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