APOPTRAK™ is a small molecule (442 Da) belonging to the anthraquinone family.It is optimally excited at 633 or 647 nm and emission in the far-red/near infra-red (NIR) peaking at 700 nm.
APOPTRAK™ is a useful dye in flow cytometry applications:
APOPTRAK™ benefits from all the advantageous "far-red" spectral properties of its parent, but differs significantly in its rate of entry into LIVE cells. APOPTRAK™ enters LIVE cells slowly and apoptotic cells rapidly, allowing a positive discrimination between these two populations. Key benefits include:-
APOPTRAK™ can bring significant benefits to your application:
in FLOW CYTOMETRY:APOPTRAK™ enters LIVE cells slowly and leaky (apoptotic) cells rapidly, to give a signal intensity difference of between 5- and 10-fold, depending on the cell type and treatment. This allows a positive discrimination between these two populations as shown in the example data below.
Published data (Wiltshire et al, Cytometry 2000) suggests an ability of APOPTRAK™ to discriminate early and late apoptotic cells when used in combination with Annexin V. The example below shows the uptake of APOPTRAK™ in a B cell lymphoma sample carrying a low burden of apoptotic cells. Cells were co-stained with FITC-Annexin V.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn