贺南京中医药大学应用PriCells产品/技术服务发表文章

 

IntegratingmicroRNAandmRNAexpression profilesinresponsetorADIation-inducedinjuryin ratlung

 

 

LingXie,JundongZhou,ShuyuZhang,QingChen,RenshengLai,WeiqunDing,ChuanJunSong,XingJunMengandJinchangWu

 

¹NanJingMedicalUniversity,AffiliatedHospitalofNanjingUniversityof ChineseMedicine,Nanjing210029,China

² DepartmentofPathology,TheAffiliatedHospitalofChineseMedicine UniversityofNanjing,Nanjing210029,China

³ SchoolofRadiationMedicineandPublicHealth,MedicalCollegeofSoochow University,Suzhou215123,China

⁴ TheCoreLaboratoryofSuzhouCancerCenterandDepartmentofRadiotherapy ofSuzhouMunicipalHospital,Suzhou215001,China

⁵ DepartmentofPathology,UniversityofOklahomaHealthScienceCenter, OklahomaCity,OKC,USA

 

 

Abstract

Purpose:Exposuretoradiationprovokescellularresponses,whicharelikelyregulatedbygeneexpressionnetworks.MicroRNAsaresmallnon-codingRNAs,whichregulategeneexpressionbypromotingmRNAdegradationorinhibitingproteintranslation.TheexpressionpatternsofbothmRNAandmiRNAduringtheradiation-inducedlunginjury(RILI)remainlesscharacterizedandtheroleofmi-RNAsintheregulationofthisprocesshasnotbeenstudied.ThepresentstudysoughttoevaluatemiRNAandmRNAexpressionprofilesintheratlungafterirradiation.

 

Methodsandmaterials:MaleWistarratsweresubjectedtosingledoseirradiationwith20Gyusing6MVx-raystotherightlung.(Adoserateof5Gy/minwasapplied).Ratsweresacrificedat3,12and26weeksafterirradiation,andmorphologicalchangesinthelungwereexaminedbyhaematoxylinandeosin.ThemiRNAandmRNAexpressionprofileswereevaluatedbymicroarraysandfollowedbyquant-itativeRT-PCRanalysis.

 

Results

ACDNAmicroarrayanalysisfound2183transcriptsbeingup-regulatedand2917transcriptsdown-regulated(P<=0.05,>=2.0foldchange)inthelungtissuesafterirradiation.Likewise,amiRNAsmicroarrayanalysisindicated15miRNAspeciesbeingup-regulatedand8down-regulated(P<= 0.05). Subsequent bioinformatics anal -yses of the differentially expressed mRNA and miRNAs revealed that alterations in mRNA expression following irradiation were negatively correlated with miRNAs expression.

 

Conclusions

OurresultsprovideevidenceindicatingthatirradiationinducesalterationsofmRNAandmiRNAexpressioninratlungandthatthereisanegativecorrelationofmRNAandmiRNAexpressionlevelsafterirradiation.Thesefindingssignificantlyadvanceourunderstandingoftheregulatorymechanismsunderlyingthepathophysiologyofradiation-inducedlunginjury.Insummary,RILIdoesnotdevelopgraduallyinalinearprocess.Infact,differentcelltypesinteractviacytokinesinaverycomplexnetwork.FurThermore,thisstudysuggeststhatmicroRNAsmayserveanimportantroleinthepathogenesisofRILIandthatunderstandingtheirroleinRILImayhaveasignificanteffectonpatientmanagementanddiagnosisinthefuture.

 

RAT-CELL-0003，PriCells