Deprecated: Required parameter $cat_id follows optional parameter $type in /www/wwwroot/ebimall.com/systems/hong.php on line 2088

Deprecated: Required parameter $where follows optional parameter $tree_id in /www/wwwroot/ebimall.com/systems/hlb.php on line 3505
Clot Retraction Test188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
您好,欢迎您进入188进口试剂采购网网站! 服务热线:4000-520-616
蚂蚁淘商城 | 现货促销 | 科研狗 | 生物在线

Clot Retraction Test

OUTLINE
Abnormal Clot Retraction indicates on abnormal platelet numbers, abnormal glycoprotein amount or structure, abnormal platelet signaling, abnormal fibrinogen levels, or abnormal fibrinogen structurePROTOCOL
  1. Use either the whole blood or platelet-rich plasma (PRP)
  2. To obtain PRP, collect 500 µl of blood on 50 µl of ACD
  3. Add 600 µl of PBS, centrifuge once at 10ѓC, 1200 rpm ( Joan, GR 412)
  4. In Glass Centrifuge Tube add 100 µl of PRP and 160µl of clot retraction buffer, and 50µl of working solution of thrombin (8 U/mL) and 5-10 µL of erythrocytes (for visualization). Vortex briefly.
  5. Place the Dead End Glass Rod (use Pasteur Pipette) to the bottom of the tube. Place the tube at 37ѓC. Check the CLOT FORMATION (appearance) and RETRACTION. Measure the volume of remained solution after clot retraction, notice the color of solution, take digital picture.
SOLUTIONS
  1. Clot Retraction Buffer: 400 mL of PBS x1 + 10 mL of PBS-MgCl2 (MgCm2=4g/L) + 10 mL PBS-CaCl2 (CaCl2= 5.3 g/L)
  2. Working Solution of Thrombin = 7 µl of Thrombin (1250U/mL) + 1 mL of Clot Retraction Buffer
ADDITIONAL INFOThe Clot Retraction Test may be used for screening of new anti-platelet drugs, anti-platelet-antibodies, or unexplained bleeding-problems. This test does not indicate exactly what is the problem but shows the direction to look for it.
REFERENCES
  1. Platelet Protocols. Research and Clinical Laboratory Procedures. McCabe White M. and Jennings L.K. Academic Press, 1999
  2. Azam M. et al, Molecular Cellular Biology, March 2001, p. 2213-2230, Vol. 21, No. 6
  3. Hodivala-Dilke KM et al, Beta3-integrin-deficient mice are a model for Glanzmann thrombasthenia showing placental defects and reduced survival. J. Clin. Investig. 103:229-238


新闻动态
行业前沿
技术文章
最新产品

188进口试剂采购网 www.188bio.cn -中国试剂网,试剂网,化学试剂网,国药试剂,抗体公司,试剂公司,试剂盒公司,苏州试剂公司,北京化学试剂公司,天津化学试剂,试剂商城,试剂代理,流式抗体 细胞库查询 sitemap