ImmunoCult™-XF T Cell Expansion Medium, Serum-Free, Xeno...

Figure 2. ImmunoCult -XF T Cell Expansion Medium Supports Greater T Cell Expansion Than Other Serum-Free and Serum-Supplemented Media

T cells were isolated from human peripheral blood samples using the EasySep Human T Cell Isolation Kit (Catalog #17951), stimulated with ImmunoCult Human CD3/CD28/CD2 T Cell Activator (Catalog #10970), and cultured in (A) ImmunoCult -XF T Cell Expansion Medium or serum-free competitor media with rhIL-2 in three replicate cultures per donor, or cultured in (B) ImmunoCult -XF T Cell Expansion Medium or serum-supplemented competitor media with rhIL-2 in three replicate cultures per donor. T cells were stimulated with ImmunoCult Human CD3/CD28/CD2 T Cell Activator on day 0 and every 7 to 8 days for the duration of the culture. T cells were analyzed on day 21 for fold expansion relative to the initial cell seeding density.
(A) Compared to all serum-free competitor media tested, ImmunoCult -XF T Cell Expansion Medium showed significantly higher expansion of total T cells. Competitors 1 to 6 represent serum-free competitor media, which include, in no particular order, X-VIVO 15 (Lonza), AIM V Medium (Life Tech), CellGro DC Medium (CellGenix), CTS OpTmizer T Cell Expansion SFM (Life Tech), TexMACS Medium (Miltenyi), and PRIME-XV T Cell Expansion XSFM (Irvine Scientific). Each column with error bars represents the mean S.E.M. (p 5x10-13 for ImmunoCult -XF T Cell Expansion Medium versus all other serum-free media, tested using the linear mixed effect model with linear regression, n = 4 to 19 donors).
(B) Compared to all serum-supplemented competitor media tested, ImmunoCult -XF T Cell Expansion Medium showed similar or significantly higher expansion of total T cells. Competitors 1 to 4 represent serum-supplemented competitor media, which include, in no particular order, X-VIVO 15 + serum, CTS OpTmizer T Cell Expansion SFM + serum, RPMI 1640 + serum, and IMDM + serum. Each column with error bars represents the mean S.E.M. (p