Product name | C5a MicroVue™ Quidel®Measurement of terminal complement pathway activation in experimental samples |
Cat-Nr. | A025 |
Range | 0.1 - 1 ng/ml |
Sensitivity | LOD: 0.01 ng/ml; LLOQ: 0.050 ng/ml |
Incubation time | 2 hours 15 minutes |
Sample volume | 20 µl (dilute 1:20 for plasma); 10 µl (dilute 1:50 for serum) |
Sample type | Serum, EDTA- and citrated plasma. |
Sample preparation | The proper collection, storage and shipment of specimens are essential. Test immediately or stored up to 4 hours at 2-8 ºC or on ice. For longer-term storage the samples should kept frozen at -70 ºC with stabilizing solution (Cat. No. A9576), dilute samples 1:1 with stabilizing solution. |
Reference values | EDTA Plasma 0.37 – 74.33 ng/ml Serum 13.37 – 179.23 ng/ml |
Species | Human |
Specificity | C5a and C5a des-Arg |
Tests | 96 |
Method | ELISA |
Intended use | C5a is generated as a result of cleavage of the terminal complement protein C5,during activation of the complement system via the classical, alternative or lectin pathway. C5a is a low molecular weight (approximately 9 kD) protein fragment of 74 amino acids. C5 a is rapidly metabolized by the serum enzyme carboxypeptidase to more stable, less active, 73 amino acid form, C5a des-Arg. Research has associated elevated levels of fluid phase and adsorbed C5a with hemo-incompatibility of some biomaterials, particularly in extracorporeal circuits. Levels of C5a have also been associated with pathogenesis of a variety of disease states, including myocardial infarction, stroke, as well as vascular leak syndrome and associated kidney injury. The role of C5a in the pathogenesis of malaria and other infectious diseases, as well as sepsis, is likewise well documented. |
Product informations | Kit Instructions (PDF 907,3 kB) Cross-reaction all species (PDF 74,6 kB) Monitoring Complement Activation SC5b-9, C5a &CH50 (PDF 99,7 kB) Measurement of Complement Activation in Human Disease (PDF 4,6 MB) Hemocompatibility (PDF 6,4 MB) Information (PDF 1,1 MB) |
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