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All our gyrase subunits are supplied at a concentration of 1 mg/ml unless otherwise specified and are purified to > 95% as assessed by SDS-PAGE. We have an extensive collection of mutants not listed here so please contact us for a full list. Different quantities and concentrations can be supplied on request.
Click on the product headings below to view details and order online.
For studies involving mutant drug-resistant E. coli gyrase subunits the wild-type subunits are available separately in the following amounts. The subunits are supplied at a concentration of 1 mg/ml, purified to > 95% as assessed by SDS-PAGE. Other quantities and concentrations are available as are the individual subunits from S. aureus and M. tuberculosis gyrases; please enquire for further information.
For structural studies the following E. coli gyrase domains are available: GyrB43, GyrB24 and GyrA59. Domains supplied at a concentration of 1 mg/ml purified to > 95% as assessed by SDS-PAGE. (see gel below for typical levels of purity)
Larger quantities/different concentrations are available, please enquire.
We offer various quinolone- and coumarin-resistant topoisomerase mutants includingE.coli, S.aureus and other organisms. The proteins can be supplied as separate subunits or as reconstituted enzyme. Typically the quinolone-resistant mutants confer 50-100 X more resistance to fluoroquinolones than the wild-type. We have more than 25 mutants in different topoisomerases and can make other mutants as requested.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn