PeproTech 2016年4月发布重组人ROR1等2种新产品！

1.重组人Mesothelin
产品描述：Originallyidentifiedasadifferentiationantigenofmesotheliomas,ovariancystadenocarcinomas,andpancreaticadenocarcinomas,mesothelinisaglycosylphosphatidylinositol(GPI)-anchored,cell-surfaceglycoproteinpredominantlysecretedbycellsofthemesothelium.Althoughmesothelinisexpressedatrestrictedlevelsbynormalmesothelialcellsofthepleural,pericardial,andperitonealmembranes,aberrantexpressionhasbeendocumentedintheaforementionedcancers,aswellasinendometrioiduterineadenocarcinomasandsquamouscellcarcinomasoftheesophagus,stomach,lung,andcervix.Proteolyticcleavageofmesothelinyieldsasoluble,polypeptidefragment-designatedmegakaryocyte-potentiatingfactor(MPF)basedonitsABIlitytostimulatemegakaryocytecolony-formingactivityofmurineIL-3inmurinebonemarrowcellcultures.OriginallyisolatedfromtheHPC-Y5pancreaticcellline,MPFhasbeensuggestedtoplayaroleintheproliferationanddifferentiationofmegakaryoctyes,andtheregulationofresultantplateletproduction.WhiletheBIOLOGicalfunctionsofbothmesothelinandMPFremainspeculative,highlevelsofexpressionincanceroustissues,comparedtolimiteddistributioninnormaltissues,stronglysuggeststheirinvolvementintumOrigenesis.Bothhavebeendemonstratedtopromotetumorcellproliferation,migration,anchorage-independentgrowth,andtumorprogression,demonstratingtheirinvolvementinheterotypiccelladhesionandthemetastaticspreadofcancer.PeproTech’sCHOcell-derivedRecombinantHumanMesothelinisaglycoproteincontaining327aminoacidresidues,andhasacalculatedmolecularweightofapproximately36.4kDa.Asaresultofglycosylation,RecombinantHumanMesothelinmigrateswithanapparentmolecularmassofapproximately40-45kDabySDS-PAGEgel,underreducingandnon-reducingconditions.

2. 重组人sIL-4Receptorα

产品描述：IL-4cansignalthroughtypeIandtypeIIreceptorcomplexes,whichshareacommongammachain(γc). ThetypeIreceptorcontains,inadditiontotheγc,anIL-4Rαsubunit,whereasthetypeIIreceptorcontainstheIL-13Rα. ThesecretedextracellulardomainofIL-4Rα,calledsIL-4Rα,bindsIL-4andantagonizesitsactivity. ItplaysanimportantroleinregulatingthedifferentiationofnaïveCD4+TcellsandclassswitchingtoIgG1andIgE. PeproTech"sCHOcell-derivedRecombinantHumansIL-4Receptorαisa23.9kDaglycoproteincorrespondingto209aminoacidresiduesoftheextracellulardomainofIL-4Rα.Asaresultofglycosylation,RecombinantHumansIL-4Receptorαmigrateswithanapparentmolecularmassofapproximately50-65kDabySDS-PAGEgel,underreducingconditions.

3. 重组人sIL-6Receptorα

产品描述：IL-6mediatesitsbiologicaleffectsthroughthetypeIIL-6receptorsystemthatconsistsoftwochains,IL-6Rαandgp130. WhiletheIL-6RαchainisthebindingcomponentspecifictoIL-6,thegp130chainonlytransmitssignalsofIL-6whenboundtoIL-6Rα. Thegp130canalsotransmitsignalsfromLIF,OSM,CNTF,IL-11andCT-1inconjunctionwithotherreceptorsubunits. Thelow-affinitybindingsiteforIL-6iscomposedofIL-6Rαalone. IL-6Rαisexpressedinawiderangeofcells,includingTcells,fibroblastsandmacrophages. SolubleIL-6Rα,whichconsistsofonlytheextracellulardomainoftheIL-6Rαchain,actsasanagoNISTofIL-6activityatlowconcentrations.PeproTech"s CHOcell-derivedRecombinantHumansIL-6Receptorαisa37.9kDaglycoproteincorrespondingto339aminoacidresiduesoftheextracellulardomainofIL-6Rα.Asaresultofglycosylation,RecombinantHumansIL-6Receptorαmigrateswithanapparentmolecularmassofapproximately57-70kDabySDS-PAGEgel,underreducingconditions.

4. 重组人LeptinReceptor

产品描述：Encodedfor,alongwithleptin,bythe obese(ob)gene,leptinreceptorisasingle-transmembrane-domainproteinoftheTypeI,orClassI,CytokineReceptorFamily.Thefulllengthisoform,OB-Rb,ishighlyexpressedinhypothalamicneurons,T-cells,andthevascularendometrium,andisthoughttobetheonlyisoformcapableoftransducingintracellularsignals.IsoformOB-Ra,whichiswidelydistributedatvaryinglevelsofexpression,demonstratesweaksignalactivityandhasbeenimplicatedintheactivetransportofleptinacrosstheblood-brainbarrier.Throughligand-bindingwithleptinreceptorandthesubsequentJAK2/STAT3signalingcascade,theADIpose-derivedcytokineleptinfunctionstosuppressappetiteandincreaseThermogenesis.Leptinandleptinreceptorhavealso,morerecently,beenimplicatedintheregulationorimmunefunction,reproduction,glucosehomeostasis,bonemetabolism,woundhealing,hematopoiesis,andangiogenesis.Mutationsofthe ob gene,whichcanresultinleptinresistanceandthedown-regulationofligandand/orreceptorexpression,havebeenconnectedtoobesityandhypothalamicpituitaryfunctioninvarious invivo models,includinghuman,mouse,andrat.PeproTech’sRecombinantHumanLeptinReceptorisasingle,glycosylatedpolypeptidechainof818aminoacidresidues,correspondingtothehumanOB-Reisoform,andhasacalculatedmolecularweightofapproximately93.5kDa.Asaresultofglycosylation,RecombinantHumanLeptinReceptormigrateswithanapparentmolecularmassofapproximately130-150kDabySDS-PAGEgel,underreducingandnon-reducingconditions.


5. 重组人CTLA-4Fc

产品描述：CTLA-4andCD28arereceptorsoftheimmunoglobulinsuperfamilythatareexpressed,alongwiththetransmembraneglycoproteinsB7-1andB7-2,byantigen-presentingcells,andwiththeseligandsconstitutecrucialco-stimulatorypathwaysforTandBcellregulatoryresponses.ItisthroughengagementwithCD28andCTLA-4thattheB7familyligandsB7-1andB7-2playprincipalrolesinimmunitybyactivatingimmuneresponseandmaintainingimmunetolerance.Co-stimulatorysignalsgeneratedbyB7-1andB7-2interactionswithCD28servetostimulateTcellactivationandpreventanergythroughtheamplificationofTcellreceptor(TCR)signaling.Incontrast,interactionsoftheligandswithCTLA-4servestomaintainTcellhomeostasisandself-tolerancethroughthedisruptionofstimulatorysignalingfromB7isoform-boundCD28complexes,andbyinducingpowerfulinhibitorysignalsinTcells.CTLA-4,likeB7-1,isonlypoorlyexpressedonrestingdendriticcells;therefore,up-regulationoftheirinteractionandresultantamplificationandregulationofTcellactivityatperipheralinflammationsitesisconsiderablydelayeduponimmuneactivation.Conversely,B7-2andCD28areconstitutivelyexpressedbyrestinghematopoieticandTcells,respectively,andasaresultareabletorapidlyinduceup-regulationuponimmuneactivation,makingthemcriticaltotheearlyco-stimulatorysignalingofimmuneresponse.UnlikeB7-1andB7-2,theligandsPD-L1(orB7-H1)andB7-H2,whichalsobelongtotheB7family,havenotbeenshowntoinfluenceimmunitythroughinteractionwithCTLA-4.B7-H2hasbeenshowntohaverestrictedinteractionwithCD28.ThedifferenceinexpressionofB7-1,B7-2andB7-H2mayenabletemporallyandspatially-specificregulationofTcellresponsethroughnon-competitiveCD28interaction.PeproTech’s CHOcell-derivedRecombinantHumanCTLA-4Fcisaglycosylated,disulfide-linkedhomodimerof714aminoacidresidueswhosemonomerconsistsofthe124-amino-acidlengthextracellularportionofCTLA-4fusedtothe231-amino-acidlengthFcportionofhumanIgG1bytwoglycines.ThecalculatedmolecularweightofRecombinantHumanCTLA-4Fcdimeris78.7kDa;however,duetoglycosylation,themonomeranddimermigrateatapparentmolecularweightsofapproximately45–50kDaand80–90kDabySDS-PAGEanalysisunderreducingconditions.

6. 重组人sCD28Fc

产品描述：CTLA-4andCD28arereceptorsoftheimmunoglobulinsuperfamilythatareexpressed,alongwiththetransmembraneglycoproteinsB7-1andB7-2,byantigen-presentingcells,andwiththeseligandsconstitutecrucialco-stimulatorypathwaysforTandBcellregulatoryresponses.ItisthroughengagementwithCD28andCTLA-4thattheB7familyligandsB7-1andB7-2playprincipalrolesinimmunitybyactivatingimmuneresponseandmaintainingimmunetolerance.Co-stimulatorysignalsgeneratedbyB7-1andB7-2interactionswithCD28servetostimulateTcellactivationandpreventanergythroughtheamplificationofTcellreceptor(TCR)signaling.Incontrast,interactionsoftheligandswithCTLA-4servestomaintainTcellhomeostasisandself-tolerancethroughthedisruptionofstimulatorysignalingfromB7isoform-boundCD28complexes,andbyinducingpowerfulinhibitorysignalsinTcells.CTLA-4,likeB7-1,isonlypoorlyexpressedonrestingdendriticcells;therefore,up-regulationoftheirinteractionandresultantamplificationandregulationofTcellactivityatperipheralinflammationsitesisconsiderablydelayeduponimmuneactivation.Conversely,B7-2andCD28areconstitutivelyexpressedbyrestinghematopoieticandTcells,respectively,andasaresultareabletorapidlyinduceup-regulationuponimmuneactivation,makingthemcriticaltotheearlyco-stimulatorysignalingofimmuneresponse.UnlikeB7-1andB7-2,theligandsPD-L1(orB7-H1)andB7-H2,whichalsobelongtotheB7family,havenotbeenshowntoinfluenceimmunitythroughinteractionwithCTLA-4.B7-H2hasbeenshowntohaverestrictedinteractionwithCD28.ThedifferenceinexpressionofB7-1,B7-2andB7-H2mayenabletemporallyandspatially-specificregulationofTcellresponsethroughnon-competitiveCD28interaction.PeproTech’s CHOcell-derivedRecombinantHumansCD28Fcisaglycosylated,disulfide-linkedhomodimerof734aminoacidresidueswhosemonomerconsistsofthe134-amino-acidlengthextracellularportionofCD28fusedtothe231-amino-acidlengthFcportionofhumanIgG1bytwoglycines.ThecalculatedmolecularweightofRecombinantHumansCD28Fcdimeris82.4kDa;however,duetoglycosylation,themonomeranddimermigrateatapparentmolecularweightsofapproximately60-70kDaand150–160kDabySDS-PAGEanalysisunderreducingconditions.
 

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