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AG Scientific/Dithiothreitol (DTT)/100 g/C-1029-100 g

More Information
Alternate Name/Synonyms
DTT; Dithiothreitol; DL-Dithiothreitol solution; (±)-threo-1,4-Dimercapto-2,3-butanediol solution
SKU
C-1030
CAS #
3483-12-3
Chemical Name
(2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol
Chemical Formula
C4H10O2S2
Molecular Weight
154.25 Da
Appearance
Clear colorless solution
Purity
≥99%
Solubility
Clear and colorless in water
USE
DTT is used as a reducing or "deprotecting" agent for thiolated DNA. The terminal sulfur atoms of thiolated DNA have a tendency to form dimers in solution, especially in the presence of oxygen. Dimerization greatly lowers the efficiency of subsequent coupling reactions such as DNA immobilization on gold in biosensors. Typically DTT is mixed with a DNA solution and allowed to react, and then is removed by filtration (for the solid catalyst) or by chromatography (for the liquid form). The DTT removal procedure is often called "desalting." Generally, DTT is used as a protecting agent that prevents oxidation of thiol groups.
Handling
Hygroscopic. Protect from moisture. Protect from light. The pharmacological and toxicological properties of this product have not been fully investigated. Use caution when handling. Do not use this compound if you are not fully trained or are unaware of the hazards involved.
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