JPT/NB Histone Peptide H2A 1:17 K5me2 (H2A.11BE1D1H1J)/SP-His_nb_007/

NBHistonePeptideH2A1:17K5me2(H2A.11BE1D1H1J)

ProductCode:SP-His_nb_007

SyntheticHistonepeptidecarrying1modification(K5me2)derivedfromhumanHistoneH2A.11BE1D1H1J(Swiss-ProtIDs:P0C0S8,P20671,P04908,Q99878,Q96KK5).
Aminoacidsequence:H-SGRG-Lys(Me2)-QGGKARAKAKTR-NH2
Amount:1vialcontaining30nmol(approx.100µg)
Purity:>70%(HPLC-MS)
Deliveryformat:Freeze-driedinambertypeglassvials
Application(s):Enzymekinetics,SubstrateSpecifity,Substrateidentification,Competitionexperiments,Co-crystallization,NMRstudies
Indication(s)/Topic(s):Cancer,Kinase,Methyltransferase,Acetyltransferase,Histones,Phosphatase,Generegulationandexpression,Epigenetics,Cellsignaling,Nucleosome
Deliverytime:10-12days

JPT"sNon-BiotinylatedHistoneCodePeptides
Inadditiontoourbiotinylatedhistonepeptides,JPToffersanon-biotinylatedlibraryofsyntheticposttranslationallymodifiedandunmodifiedhumanhistonepeptidesthatisnowavailableasindividualpeptides.Thepeptidelibraryconsistsof160peptidesfromdifferenthistonefamilies.PeptidescarryPTMsatreportedsites(e.g.acetylation,methylation,phosphorylation...)invariouscombinationsrepresentingnaturalvariants.
JPT"shighlyskilledandcommittedscientificstaffensuresthatthemostappropriatemethodsandtechniquesareselectedforeverysynthesisproject.AllofJPT"scatalogpeptidesareprovidedwithHPLC-MSanalysestoconfirmtheidentityanddemonstratethehighqualityofourpeptides.

BenefitsofJPT"sHistoneCodePeptides
-WholeproductionperformedinaregulatedEuropeanenvironmentaccordingtoDINENISO9001:2015&GCLPstandards;auditswelcome!
-Forhigh-throughputscreeningJPT"sHistoneCodePeptideMicroarrayisavailabledisplayingmorethan3000peptides.
-ProvisionoffreezedriedaliquotsforenhancedstABIlity
-Proventrackrecordformanyapplications

NBHistonePeptideH2A1:17K5me2(H2A.11BE1D1H1J)

SelectedReferencesforHistonePeptideTools
"HistoneH2AandH4N-TerminalTailsarePositionedbytheMEP50WD-RepeatProteinforEfficientMethylationbythePRMT5ArginineMethyltransferase"
Burgosetal.,J.Biol.Chem.(2015)-PMID:25713080
"H3K23me2isaNewHeterochromaticMarkinCaenorhaBDitiselegans"
Vandammeetal., NucleicAcidsRes.(2015)-PMID:26476455
"StructureoftheArginineMethyltransferasePRMT5-MEP50RevealsaMechanismforSubstrateSpecificity"
Hoetal.PLOSONE(2013)-PMID:23451136
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal,ApplicationNote(2013)(fulltext)
"ComprehensivePeptideMicroarraysforHistoneResearch"
Schnatbaumetal,32thEPS(2012)(fulltext)

ApplicationNotes
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal.ApplicationNote(2013)(fulltext)
"ComprehensiveCharacterizationofAntibodiesDirectedtowardsEpigeneticHistone-Modifications"
Maschetal.ApplicationNote(2015)(fulltext)

TestimonialforHistoneCodePeptideMicroarray
"JPT"snewextremelyhigh-densityhistonepeptidearrayisadramaticleapforwardforanalysisofthehistonecodehypothesis.Thecompletesequencecoverageacrosscorehistonesandhistonevariantsaswellasthepresenceofcommonandrarehistonepost-translationalmodifications "aloneandincombination"makesthisanunparalleledtoolforstudyingthe"writers"and"readers"ofthehistonecode.MylaboratoryhasusedthesearraystoaskthesequenceandPTMdependenceofahistonewriterenzymeandwehavediscoveredanactivityspecifyingcodethatwillkeepusbusyforyearstocome."
DavidShechter,PhD,DepartmentofBiochemistry,AlbertEinsteinCollegeofMedicine,YU(NY,USA)