CST/Organelle Localization IF Antibody Sampler Kit #8653/8653T

Kit Usage Information

Protocols

• 3288:Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence
• 3501:Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence*
• 5318:Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Immunofluorescence
• 9091:Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence*, Flow Triton Permeabilization (Rabbit)
• 12290:Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Flow

Product Description

The Organelle Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immunocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or two western blots with each antibody.

Specificity / Sensitivity

Each antibody in the Organelle Localization IF Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein, or with a synthetic peptide corresponding to residues surrounding Ala520 of human AIF protein, residues surrounding Ser70 of human EEA1 protein, the sequence of human PDI protein, or residues surrounding Gly147 of human RCAS1 protein.

Background

Knowledge of the subcellular location of a protein may reveal its potential role in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.

Pathways & Proteins

Explore pathways + proteins related to this product.

Database Links

Swiss-Prot Acc.:Q15075, P11279, O95831, P07237, O00559

Entrez-Gene Id:8411, 3916, 9131, 5034, 9166

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