CST/Polycomb Group 2 (PRC2) Antibody Sampler Kit #62083/62083T

Kit Usage Information

Protocols

• 3737:Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq
• 5246:Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq
• 7074:Western Blotting
• 13594:Western Blotting, Immunoprecipitation (Magnetic), ChIP Magnetic, Chromatin IP-seq
• 14129:Western Blotting, Immunoprecipitation (Agarose), ChIP Magnetic, Chromatin IP-seq
• 42088:Western Blotting, Immunoprecipitation (Magnetic)
• 51673:Western Blotting

Product Description

The Polycomb Group 2 (PRC2) Antibody Sampler Kit provides an economical means of evaluating total levels of Polycomb Group 2 Proteins. The kit contains enough primary and secondary antibodies to perform two western blot experiments.

Specificity / Sensitivity

Each antibody in the Polycomb Group 2 (PRC2) Antibody Sampler Kit detects endogenous levels of target proteins.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Arg354 of human Ezh2, Pro257 of human SUZ12 protein, Leu23 of human EED, Asp1114 of human JARID2, Leu345 of human AEBP2, and the carboxy terminus of EZH1.

Background

The polycomb group (PcG) proteins are involved in maintaining the silenced state of multiple developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1-4). Enhancer of zest homolog 1 (Ezh1) and enhancer of zest homolog 2 (Ezh2) are members of this large protein family and are subunits of the polycomb repressor complex 2 (PRC2), also known to contain SUZ12, EED, JARID2, and AEBP2. Ezh1 and its paralog Ezh2 are mutually exclusive catalytic subunits of the PRC2 complex, which functions to mono-, di-, and tri-methylate Lys27 on histone H3, all marks that are associated with transcriptional repression. SUZ12 and EED proteins are also absolutely required for methyltransferase activity (5). JARID2 and AEBP2 are both accessory proteins that function to recruit the PRC2 complex to target genes and enhance methyltransferase activity by binding to DNA and histone proteins in nucleosomes (6-14).

1. Boyer, L.A. et al. (2006) Nature 441, 349-53.
2. Cao, R. et al. (2002) Science 298, 1039-43.
3. Müller, J. et al. (2002) Cell 111, 197-208.
4. Lee, T.I. et al. (2006) Cell 125, 301-13.
5. Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.
6. Peng, J.C. et al. (2009) Cell 139, 1290-302.
7. Shen, X. et al. (2009) Cell 139, 1303-14.
8. Pasini, D. et al. (2010) Nature 464, 306-10.
9. Li, G. et al. (2010) Genes Dev 24, 368-80.
10. Son, J. et al. (2013) Genes Dev 27, 2663-77.
11. Kaneko, S. et al. (2014) Mol Cell 53, 290-300.
12. Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.
13. Kim, H. et al. (2009) Nucleic Acids Res 37, 2940-50.
14. Kalb, R. et al. (2014) Nat Struct Mol Biol 21, 569-71.

Pathways & Proteins

Explore pathways + proteins related to this product.

Database Links

Swiss-Prot Acc.:Q6ZN18, O75530, Q92800, Q15910, Q92833, Q15022

Entrez-Gene Id:121536, 8726, 2145, 2146, 3720, 23512

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