Illumina/RiboZero rRNA Removal Kit (H/M/R) 6 reactions/MRZH116/1 Ea

• RemovesribosomalRNA(rRNA)inasinglepass
• SequencingdatacontainscompletetranscriptomeofcodingandnoncodingRNAspecies
• Goodforintactandpartiallydegradedsmallsamples–only1μgoftotalRNArequired

Sequencingisanimportantdiscoverytoolforresearchintodisease,development,andmore.ThesequencingdatafromsamplestreatedwithRibo-Zero(Human/Mouse/Rat)isfocusedonvaluableRNAspecies,tofacilitatediscoveryofrelevantgenes,splicevariantsandisoforms.

Ribo-Zero(Human/Mouse/Rat)offersinformativesequencingresultsbyremovingunwantedrRNApriortosequencing.Thiskitremovescytoplasmic(nuclear-encoded)rRNAs.

Ribo-Zeroretainsthecompletetranscriptome

Ribo-ZeroremovesrRNAinordertoretaintheregulatoryregionsofnoncodingRNA,evenfromlow-qualitysamples.Othermethods,suchasPoly(A)depletion,donotretainnoncoding,regulatoryinformation.

OverviewoftheRibo-Zeroprocess

VirtuallyallrRNAisdepletedfromintact,degraded,andbadlydegraded(FFPE)samplesin2hours.

Step1:Washthemagneticbeads
Step2:TreatsampleswithrRNARemovalSolution
Step3:RemoverRNA
Step4:PurifyrRNA-depletedsamples,andproceedwithlibrarypreparation

Alternativekitversions

TheRibo-ZeroGold(Human/Mouse/Rat)rRNARemovalKitremovesbothcytoplasmicandmitochondrialrRNAs,andiscompatiblewithlowinputsamples(e.g.100ngoftotalRNA).

Relatedinformation

rRNARemovalKitSelectionGuide
Ribo-ZeroKitSpeciesCompatibility
Ribo-ZeroKitFAQs