5-trisphosphatereceptorExpressionandroleofinositol1,4,5-trisphosphatereceptorandryanodinereceptorinahumanlensepithelialcellline人晶状体上皮细胞系内肌醇1,4,5-三磷酸受体和兰尼碱受体的表达和作用短句来源
相似匹配句对(5)itcouldimprovemicro-circulation(5)短句来源5.Intheexperimentoftemperature,relativeM.aeruginosa,thecompetitivepredominanceofS.quadricaudaandN.5..短句来源三.三.短句来源Biosynthesisof5"-nucleosidestriphosphatebyimmobilizedbeeryeastcells利用固定化啤酒酵母合成5’-核苷三磷酸短句来源Three.三.短句来源
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5-trisphosphatereceptorTemperature-dependenceandconformationalbasisofinositol1,4,5-trisphosphatereceptorregulatedbyCa2+Type1inositol1,4,5-trisphosphatereceptorknock-outmice:theirphenotypesandtheirmeaninginneuroscienceandclinicalpraElevated[3H]inositol1,4,5-trisphosphatebindingsitesandexpressedinositol1,4,5-trisphosphatereceptorproteinlevelinplaIontophoreticNMDAresponsesareenhancedinthepresenceofACPD,aneffectblockedbyGDPβSandheparin(intracellularinositol1,4,5-trisphosphatereceptorantagonist).Thecellbodies,axonsanddendritesincludingspinybranchletsanddendriticspinesofnormalPurkinjecellswereintenselystainedbytheantibodyagainstP400glycoprotein/inositol1,4,5-trisphosphatereceptorprotein(P400/IP3R).更多
ObjectiveToinvestigatethepathwayofbasicfibroblastgrowthfactor(bFGF)inducedchangesofcalciuminahumanlensepithelialcelllineLEC-B3.MethodsThe3rdpassageofLEC-B3cellswasusedinthepresentstudies.bFGF(10μg/L),caffeine(10mmol/L),ryanodine(10mmol/L),procaine(50mmol/L)andgenistein(0.5mmol/L)wereaddedtotheculturemedium.Changesofcalciumconcentrationwereobservedandanalyzedwithlaserscanningconfocalmicroscope.ResultsbFGF(10μg/L)couldinduceaprompt...
ObjectiveToinvestigatethepathwayofbasicfibroblastgrowthfactor(bFGF)inducedchangesofcalciuminahumanlensepithelialcelllineLEC-B3.MethodsThe3rdpassageofLEC-B3cellswasusedinthepresentstudies.bFGF(10μg/L),caffeine(10mmol/L),ryanodine(10mmol/L),procaine(50mmol/L)andgenistein(0.5mmol/L)wereaddedtotheculturemedium.Changesofcalciumconcentrationwereobservedandanalyzedwithlaserscanningconfocalmicroscope.ResultsbFGF(10μg/L)couldinduceapromptincreaseofcalciumconcentrationwhencellsculturinginculturemediumwithorwithoutcalcium.Noobviousdifferenceofcalciumconcentrationcouldbeobservedbetweenthesetwoconditionsexceptthattheamplitudeofincreaseofcalciumconcentrationundercalciumfreesolutionswasslightlyhigherthanthatinmediumwithcalcium.Both50mmol/Lprocaineand10mmol/LryanodinepartiallyinhibitedthecalciumincreaseinducedbybFGF.Aftertheadditionof10mmol/Lcaffeine,10μg/LbFGFcouldnotinduceasiginificantincreaseofcalcium.Genisteinat0.5mmol/LsignificantlyinhibitedbFGFinducedchangesofcalcium.ConclusionsbFGFinducingcalciumionconcentrationincreasemainlydependsonTPKpathway.Mostofthecalciumionscomefromthereleaseofintracellularcalciumpoolsinhumanlensepithelialcell.BoththeIP3RandRyRtakepartintheaction,whileIP3Risthemoreimportantfactor.
目的初步探讨碱性成纤维细胞生长因子(bFGF)作用于人晶状体上皮细胞(LEC)系B3(LECB3)内Ca2+转导的途径。方法人LECB3传代培养,在激光扫描共焦显微镜下于解冻后的第三代细胞中分别加入10μg/LbFGF、10mmol/L咖啡因、10mmol/L兰尼碱、50mmol/L普鲁卡因及05mmol/L金雀异黄素,通过观测细胞内相对荧光强度实时观察细胞内Ca2+浓度的变化。结果10μg/LbFGF在细胞外液含或无Ca2+、Mg2+的情况下,均可迅速引起人LECB3内Ca2+浓度升高,且持续时间基本相同;而在细胞外液无Ca2+和Mg2+、含1mmol/L乙二醇双乙醚四乙酸时,人LECB3内Ca2+浓度更高。50mmol/L普鲁卡因和10mmol/L兰尼碱与10μg/LbFGF共同作用,人LECB3内Ca2+浓度虽升高,但低于10μg/LbFGF单独作用的效果;10mmol/L咖啡因和05mmol/L金雀异黄素可明显降低追加的10μg/LbFGF升高人LECB3内Ca2+浓度的作用,...
目的初步探讨碱性成纤维细胞生长因子(bFGF)作用于人晶状体上皮细胞(LEC)系B3(LECB3)内Ca2+转导的途径。方法人LECB3传代培养,在激光扫描共焦显微镜下于解冻后的第三代细胞中分别加入10μg/LbFGF、10mmol/L咖啡因、10mmol/L兰尼碱、50mmol/L普鲁卡因及05mmol/L金雀异黄素,通过观测细胞内相对荧光强度实时观察细胞内Ca2+浓度的变化。结果10μg/LbFGF在细胞外液含或无Ca2+、Mg2+的情况下,均可迅速引起人LECB3内Ca2+浓度升高,且持续时间基本相同;而在细胞外液无Ca2+和Mg2+、含1mmol/L乙二醇双乙醚四乙酸时,人LECB3内Ca2+浓度更高。50mmol/L普鲁卡因和10mmol/L兰尼碱与10μg/LbFGF共同作用,人LECB3内Ca2+浓度虽升高,但低于10μg/LbFGF单独作用的效果;10mmol/L咖啡因和05mmol/L金雀异黄素可明显降低追加的10μg/LbFGF升高人LECB3内Ca2+浓度的作用,后者尤为明显。结论bFGF主要通过激活络氨酸蛋白激酶信号转导途径引起人LECB3内Ca2+浓度升高;肌醇1,4,5三磷酸受体和兰尼碱受体通道发挥一定作用;Ca2+主要来源于细胞内Ca2+库释放。
Complexsignaltransductionnetworkshavebeenconstructedinplants.Theyarenecessaryforplantstoregulatetheirgrowthanddevelopmentalprograms,andresponsetoenvironmentalcues.Manyintracellularandextracellularsignalmoleculesparticipateintheregulationofplants.Peoplebelievedtheexistenceofextracellularpeptidesignalmoleculesinplantssincethediscoveryofextracellularcalmodulin(CaM).Becauseoftheextensivebiologicalfunctions,extracellularCaMparticipatesinnearlyallplantgrowth...
Complexsignaltransductionnetworkshavebeenconstructedinplants.Theyarenecessaryforplantstoregulatetheirgrowthanddevelopmentalprograms,andresponsetoenvironmentalcues.Manyintracellularandextracellularsignalmoleculesparticipateintheregulationofplants.Peoplebelievedtheexistenceofextracellularpeptidesignalmoleculesinplantssincethediscoveryofextracellularcalmodulin(CaM).Becauseoftheextensivebiologicalfunctions,extracellularCaMparticipatesinnearlyallplantgrowthanddevelopmentprocesses.Wesum-marizedrecentfindings,andthendelineatedextracellularCaMtransmembraneandintracellularsignalingtransduc-tionpathways.ThedirectorindirectrelationshipsamongheterotrimericGproteins,PLC-IP3-IP3Rsignalingpathway,reactiveoxygenspecies(ROS)andcalciumchannels,werethecorecontentsofextracellularCaMsignalingtransduction.
植物体需要构建复杂的信号转导体系以调节自身的生长发育过程并适应外界环境的变化,这种功能的实现需要胞内和胞外诸多信号分子的参与,胞外钙调素的发现使人们开始相信植物细胞外多肽信使的存在。胞外钙调素的生物学功能极其广泛,几乎涉及到植物生长发育的各个阶段,其信号转导途径是目前研究得最多也是最为清楚的方面,异三聚体G蛋白、磷脂酶C(PLC)-肌醇三磷酸(IP3)-肌醇三磷酸受体(IP3R)信号通路、活性氧和Ca2+通道之间直接或间接的相互作用是胞外钙调素信号转导的核心。
 
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