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Glen research/3'-dT-CE Phosphoramidite/1kit/10-1084-90E

Technical Documents
Description
Details
Dilution/Coupling Data
Related Products

Technical Documents

Safety Data Sheet

Glen Report 10.17: More Novel Monomers

Description

Cellular DNA and RNA are made up of ribo- and 2"-deoxyribonucleic acids linked together via 3"-5" phosphodiester linkages and by far comprise the bulk of polynucleic acids found in cells.Much less common are oligonucleotides which have 2"-5" linkages.However, a unique feature of 2"-5" linked oligonucleotides is their ability to bind selectively to complementary RNA.These features suggest a number of interesting uses for 2"-5" linked oligos such as their use as RNA specific probes or in antisense oligos.Recently, oligos have been synthesized using 3"-deoxy-2"-phosphoramidites and 2"-deoxy-3"-phosphoramidites to producechimeras with 2"-5" linked ends and 3"-5" linked central regions.It was found that 2"-5" phosphorothioate oligos: 1) bind selectively to complementary RNA with the same affinity as phosphodiester oligos;2) exhibit much less nonspecific binding to cellular proteins;3) do not activate RNase H. A 3"-deoxynucleoside at the 3"-terminus of an otherwise normal oligonucleotide effectively blocks polymerase extension.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
DiluentAnhydrous Acetonitrile
StorageRefrigerated storage, maximum of 2-8°C, dry
Stability24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of Additions
LV40LV20040nm0.2μm1μm10μm
10-1084-020.25 g.25grams3.3698.6759.23726.9119.734.93
10-1084-90100 µmol.074grams120127.55.4541
10-1084-9550 µmol.037grams0.53.3321.250.910.670.17

Expedite

Catalog #Pack SizeGrams/PackDilution (mL)Approximate Number of Additions
Molarity50nm0.2μm1μm15μm
10-1084-020.25 g.25grams5.010.0793.858.6342.645.86
10-1084-90100 µmol.074grams1.50.0723.614.7510.731.48
10-1084-9550 µmol.037grams0.750.078.65.383.910.54

Related Products

3'-dA-CE Phosphoramidite
3"-dA-CE PhosphoramiditeCatalog #: 10-1004
3'-dC-CE Phosphoramidite
3"-dC-CE PhosphoramiditeCatalog #: 10-1064
3'-dG-CE Phosphoramidite
3"-dG-CE PhosphoramiditeCatalog #: 10-1074
3'-dA-CPG
3"-dA-CPGCatalog #: 20-2004
3'-dC-CPG
3"-dC-CPGCatalog #: 20-2064
3'-dG-CPG
3"-dG-CPGCatalog #: 20-2074
3'-dT-CPG
3"-dT-CPGCatalog #: 20-2084
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