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| AlternativeName: | NACP,Non-Aβcomponentofsenileplaques |
| Host: | Rabbit |
| Immunogen: | Syntheticpeptidecorrespondingto[Cys]+aa117-131ofhumanα-synucleinconjugatedtoKLH.Thesequenceisconservedinratα-synucleinexceptfortwosubstitutions,namelyAsp121SerandAsn122Ser. |
| UniProtID: | P37840 |
| GenBankID: | 2136215 |
| Speciesreactivity: | Human,Rat |
| Specificity: | Thisantibodyhasbeenshowntoreactstronglywithhumanbrain-derivedα-synuclein.Theantibodyalsoreactswithratbrain-derivedα-synucleinbutreactivityappearssubstantiallylessthanwithhuman. |
| Crossreactivity: | Doesnotcross-reactwithmouseα-synuclein. |
| Applications: | IHC,WB |
| RecommendedDilutions/Conditions: | Westernblot(1:1,000-1:20,000) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| ApplicationNotes: | Westernblot:TheantibodyhasbeencharacterizedbysingledimensionSDS-PAGEusingrecombinantα-andβ-synucleinsaswellasrat,mouseandhumanbrainlysateswithandwithoutpeptideblockade.Studieswiththerecombinantproteinsdemonstratedtheabsolutespecificityoftheantibodyforα-synuclein.Theantibodygivesastrongbandatarelativemolecularweightofapproximately15kDawithhumanbrainlysatewhichiswhollyabolishedbypreincubationwiththeimmunizingpeptide(Prod.No.BML-SP9340). Immunohistochemistry:Theantibodyhasbeentestedonde-waxedtissuesectionsofhumanbrainfixedinneutralbufferedformaldehyde.Astandardprotocolshouldbefollowed. |
| BlockingPeptide: | ForBlockingpeptideseeProd.No.BML-SP9340. |
| PurityDetail: | Purifiedbycaprylicacidandammoniumsulphateprecipitation. |
| Formulation: | Liquid.InPBS,pH7.4,containing10mMsodiumazide. |
| Use/Stability: | Vialcontents,StorageandUse:Vialcontainsanimmunoglobulinpreparation,partiallypurifiedbychromatographyfromwholeserum,suspendedinphosphate-bufferedsaline(pH7.4)containing0.01Msodiumazide.Storeunopenedvialat-20°Cuntilrequiredforuse.AVOIDREPEATEDFREEZE-THAWCYCLES.Aliquotundilutedantibodyintosmallervolumes(notlessthan10μL)priortofreezingifappropriate.Theuseofhighquality‘antiserum-grade’plasticorglassvialsisrecommended.Storedilutedantibodyat2-4°C(donotfreeze)andusewithin1month.Dilutetoworkingstrengthwith50mMPBS(pH7.4)containing1.5%sodiumchlorideand1%normalgoatserum(ifagoatanti-rabbitIgGlinkerantibodyistobeused). |
| Handling: | Avoidfreeze/thawcycles.Afteropening,preparealiquotsandstoreat-20°C. |
| Shipping: | ShippedonBlueIce |
| LongTermStorage: | -20°C |
| ScientificBackground: | α-SynucleinaccumulatesinLewybodies,whichareintraneuronalcytoplasmicinclusionspresentinthebrainsofsporadicParkinsonsdiseasepatients,andisimplicatedinthepathogenesisofParkinsonsdiseaseandrelatedneurodegenerativedisorders.α-Synucleinappearstoassociatewithotherproteinsthataggregateandisfoundinβ-amyloidplaquesandneuritictanglesinAlzheimersdisease. |