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absoluteantibody/Anti-Ara h 2/Ara h 3 [PA12P3F10]/50 μg/Ab01494-20.0

UniProtAccessionNumberofTargetProtein:Q6PSU2;O82580

AlternativeName(s)ofTarget:Arah2;Arachishypogaea;peanut;Arah3;Conglutin;2Salbumin;Conglutin-7;2Sprotein1;SeedstorageproteinSSP1;SeedstorageproteinSSP2;Glycinin;Arah3Immunogen:ThisantibodywasacquiredbyisolatingIgEBcellsfromperipheralbloodofhumanindividualsallergictopeanutsandsubsequentcloningandexpressingtheantibody(crooteetal.,2018).

Specificity:ThisantibodyrecognisespredominantlyArah2,butitalsocross-reactswithArah3andweaklywithArah1.Arah2isoneofthemajorpeanutallergens.

ApplicationNotes:ELISAtestsconfirmedthatthisantibodystronglybindsArah2andtoalesserdegreeArah3(Crooteetal.,2018).BiolayerinterferometryexperimentsrevealedthatPA12P3F10antibodyexhibitsveryhighaffinitytoArah2andArah3peanutallergens(Crooteetal.,2018).Consequently,thisantibodyanditsengineeredversions(suchasanIgGversionwiththesamevariabledomainsastheoriginalIgE)mightbeusedinthedevelopmentofthetherapeuticagentsforallergies,assuchIgGscouldcompetewithallergenicIgEsfortheallergen(Crooteetal.,2018).

Antibodyfirstpublishedin:Crooteetal.High-affinityallergen-specifichumanantibodiesclonedfromsingleIgEBcelltranscriptomes.Science.2018Dec14;362(6420):1306-1309.doi:10.1126/science.aau2599.PMID:30545888Noteonpublication:ThisarticledescribesthegenerationandcharacterisationofthePA12P3F10antibody.

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