LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:r
ImmunogenPeptide(C)RPEPRGLPQ*SELNQE,correspondingtoaminoacidresidues160-174ofratα2B-adrenoceptorwithreplacementofcysteine169(C169)withserine(*S)(AccessionP19328).2ndextracellularloop.
Accession(Uniprot)NumberP19328
GeneID24174
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyRat-14/15aminoacidresiduesidentical;mouse-13/15aminoacidresiduesidentical;human-10/14aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.8mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1μgpeptideper1μgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ih,wb
Mayalsoworkin:ic,ifc,ip,lci
WesternblotWesternblotanalysisofratkidney(lanes1and5),lung(lanes2and6),liver(lanes3and7)lysatesandratskeletalmusclemembranes(lanes4and8):
1.Anti-α2B-AdrenergicReceptor(extracellular) Antibody(#AAR-021),(1:200).
2.Anti-α2B-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
ImmunohistochemistryExpressionofα2B-Adrenoreceptorinratlung
ImmunohistochemicalstainingofratlungparaffinembeddedsectionsusingAnti-α2B-AdrenergicReceptor(extracellular)Antibody(#AAR-021),(1:100).α2B-Adrenoreceptorisexpressedintherespiratoryepitheliumofthebronchioli(arrows).Hematoxilinisusedasthecounterstain.
ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORS
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
Link,R.E.etal.(1996)Science273,803.
Makaritsis,K.P.etal.(1999)Hypertension33,14.
Eason,M.G.andLiggett,S.B.(1992)J.Biol.Chem.267,25473.
Pitcher,J.A.etal.(1998)Annu.Rev.Biochem.67,653.
Kurose,H.andLefkowitz,R.J.(1994)J.Biol.Chem.269,10093.
ScientificbackgroundAdrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.
AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.
Adrenoceptorsaredividedintothreetypes:α1,α2 andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A,α1B,α1D,α2A,α2B,α2C,β1,β2,β31,2.Adrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem1,2.
Theα2B-adrenoceptorhasadistinctpatternofexpressionwithinthebrain,liverlungandkidney,andrecentstudiesusingtheknockoutmousesystemhaveshownthatdisruptionofthisreceptorindeedaffectsmouseviABIlity3,bloodpressureresponsestoα2-adrenoceptoragoNISTs3 andthehypertensiveresponsetosaltloADIng4.
Liketheα2A-adrenoceptorsubtype,theα2B-adrenoceptorundergoesshorttermagonistpromoteddesensitization5.ThisdesensitizationisduetothephosphorylationofthereceptorbyG-proteincoupledreceptorkinases(GRKs)6 whichultimatelypromotesuncouplingofthereceptorfromtheG-proteinsubunit7.
LyophilizedPowderControlAntigenIncluded
Clickheretoreceivea25µlfreetrialsample!
Lastupdate:19/02/2019
AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanextracellularepitopeoftheratα2B-adrenoceptor.Anti-α2B-AdrenergicReceptor(extracellular)Antibody(#AAR-021)canbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeα2B-adrenoceptorfrommouse,rat,andhumansamples.
Forresearchpurposesonly,notforhumanuse
WesternblotWesternblotanalysisofratkidney(lanes1and5),lung(lanes2and6),liver(lanes3and7)lysatesandratskeletalmusclemembranes(lanes4and8):
1.Anti-α2B-AdrenergicReceptor(extracellular) Antibody(#AAR-021),(1:200).
2.Anti-α2B-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
