LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:h,m,r
ImmunogenPeptideCKQGFLSSNGQNHFK,correspondingtoaminoacidresidues58-72ofhumanEMR1(Accession Q14246).Extracellular,N-terminus.
Accession(Uniprot)NumberQ14246
GeneID2015
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyMouse,Rat–11/14aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
LabelFluoresceinisothiocyanate(FITC).
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution50µldoubledistilledwater(DDW).
Antibodyconcentrationafterreconstitution1mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefortwoweeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1µgpeptideper1µgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AER-051),anddirectflowcytometry(labeledantibody).
Applications:fc
Mayalsoworkin:ic,lci
DirectflowcytometryDirectflowcytometryofliveintactmouseJ774macrophagecells:
___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).
DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:
___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).
ReferencesLegrand,F.etal.(2014)J.AllergyClin.Immunol.133,1439.
McKnight,A.J.andGordon,S.(1998)J.Leukoc.Biol.63,271.
Araç,D.etal.(2012)EMBOJ.31,1364.
ScientificbackgroundHumanepidermalgrowthfactor(EGF)–likemodulecontainingmucin-likehormonereceptor1(EMR1)isasurfacereceptorwithunknownfunctionthatbelongstotheadhesionfamilyoftheGprotein-coupledreceptors(GPCR).ThisreceptorisencodedbytheADGRE1andlikeallGPCRs,containsseventransmembranedomains,anintracellularC-terminusandanextracellularN-terminus.TheNH2domainhassixEGF-likedomains.
AdhesionGPCRssharealongN-terminalextracellularsequencescomprisingmultipledomains,separatedfromthetransmembranesegmentsbyaserine/threonine-richdomain,afeaturereminiscentofmucin-like,single-span,integralmembraneglycoproteinswithadhesiveproperties1,2.
EMR1proteinisahomologofF4/80,amurineproteinthatiswidelybeingusedasaMarkerofmurinemacrophagepopulations1.
HumanEMR1isexclusivelyexpressedonmatureeosinophilsinthebloodandbonemarrow andinnasalpolyps.Thus,EMR1is consideredtobeapotentialtherapeutictargetforthetreatmentofeosinophilicdisorders1,3.
LyophilizedPowderControlAntigenIncluded
Lastupdate:20/03/2019
Anti-EMR1(extracellular)Antibody (#AER-051)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblotandlivecellimagingapplications.Itrecognizesanextracellularepitopeandisthusidealfordetectingthereceptorinlivingcells.IthasbeendesignedtorecognizeEMR1fromhuman,mouse,andratsamples.
Anti-EMR1(extracellular)–FITCAntibody (#AER-051-F)is directlyconjugatedtofluoresceinisothiocyanate(FITC). The antibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.
Forresearchpurposesonly,notforhumanuse
DirectflowcytometryDirectflowcytometryofliveintactmouseJ774macrophagecells:
___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).
DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:
___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).
ReferencesLegrand,F.etal.(2014)J.AllergyClin.Immunol.133,1439.
McKnight,A.J.andGordon,S.(1998)J.Leukoc.Biol.63,271.
Araç,D.etal.(2012)EMBOJ.31,1364.
