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Alomone/Anti-β1-Adrenergic Receptor (extracellular) Antibody/AAR-023/0.2 ml

Cat#:AAR-023
  • LyophilizedPowder
  • AntigenIncl.
  • ShippedatRoomTemp.
    • Type:Polyclonal
    Source:Rabbit
    Reactivity:m,r
    Mayalsoworkin:h
    Immunogen
    Peptide(C)RAESDEARR(S)YNDP,correspondingtoaminoacidresidues200-214ofratADRB1 (Accession P18090).2nd extracellularloop.
    Accession(Uniprot)NumberP18090
    GeneID64508
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyRat,Mouse,human-14/15aminoacidresiduesidentical.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.85mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Controlantigenreconstitution100µldoubledistilledwater(DDW).
    Controlantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1µgpeptideper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:ih,wb
    Mayalsoworkin:ic,ifc,ip,lci
    Westernblot
    Westernblotanalysisofratbrain(lanes1and4),mousebrain(lanes2and5)andratlung(lanes3and6)membranes:
    1-3. Anti-β1-AdrenergicReceptor(extracellular) Antibody(#AAR-023),(1:200).
    4-6.Anti-β1-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
    Immunohistochemistry
    Expressionofβ1-Adrenoceptorinratandmousebrain
    Immunohistochemicalstainingofratdorsalraphenucleusandmousecerebellumusing Anti-β1-AdrenergicReceptor(extracellular) Antibody(#AAR-023),(1:400). A. β1-Adrenoceptorstaining(red) inratdorsalraphenucleusappearsinneurons(horizontalarrows). C. β1-Adrenoceptor inmousecerebellumstaining(red)isdetectedingranulecells(horizontalarrows)andinthePurkinjecelllayer(verticalarrow). B,D.NuclearstainingusingDAPIasthecounterstain(blue).
    References
    1. Gilbert,C.E.etal.(2014)Int.J.Mol.Sci.15,2929.
    2. Woo,A.Y.etal.(2012)ActaPharmacol.Sin.33,335.
    3. Vicco,M.H.etal.(2014)Can.J.Cardio.30,332.
    4. Wallukat,G.etal.(2002)Herz27,683.
    5. Kamal,F.A.etal.(2014)Circ.Res.114,742.
    Scientificbackground

    β-Adrenergicreceptors(βAR)belongtothesuperfamilyof G-proteincoupledreceptors (GPCRs).βARstimulationbythesympatheticnervoussystemorcirculatingcatecholaminesisinvolvedinperipheralbloodcirculation,metabolicregulation,musclecontraction,andcentralneuralactivities.Intheheart,acuteβARstimulationservesasthemostpowerfulmeanstoregulatecardiacoutputinresponsetoafight-or-flightsituation.

    TherearethreeβARsubtypes,β1AR, β2AR and β3AR,incardiacmyocytes1.Thereceptorsconsistofsevenmembrane-spanningdomains,threeintra-andthreeextracellularloops,anextracellularN-terminaldomain,andanintracellularC-terminaltail2.

    β1ARisthepredominantreceptorsubtypeexpressedinhumancardiomyocytes.β1ARisalsoexpressedinthekidney3.IthasbeenimplicatedinADIpogenesisandmemoryformation4.

    β1ARhasbeenwidelyconnectedwithheartdisease.Boththedownregulationandoverstimulationofβ1ARhavebeenconnectedtomyocyteapoptosis.Inaddition,chronicheartfailureischaracterizedbyabnormalitiesinβ1ARsignaltransduction.ItwasdemonstratedthatheartfailureisalsoinducIBLeinanimalmodelsbyoverexpressionofcomponentsoftheβ1ARsignaltransductioncascade.Transgenicmiceoverexpressingβ1ARdevelop,withaging,heartfailuresimilartothatobservedindilatedcardiomyopathyinhumans5.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    LyophilizedPowderControlAntigenIncluded
    receivea25µlfreetrialsample!
    Lastupdate:26/12/2018

    Anti-β1-AdrenergicReceptor(extracellular)Antibody(#AAR-023)isahighlyspecificantibodydirectedagainstanextracellularepitopetheratβ1-adrenoceptor.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.Theantibodyrecognizesanextracellularepitopeandisthusidealfordetectingthereceptorinlivingcells.Ithasbeendesignedtorecognizeβ1ARfrommouse,ratandhumansamples.

    Forresearchpurposesonly,notforhumanuse
    Westernblot
    Westernblotanalysisofratbrain(lanes1and4),mousebrain(lanes2and5)andratlung(lanes3and6)membranes:
    1-3. Anti-β1-AdrenergicReceptor(extracellular) Antibody(#AAR-023),(1:200).
    4-6.Anti-β1-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
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