产品资料

Cellgs/PODS® Human BMP-4/PODS<sup>®</sup> Human BMP-4, 1 billion/PPH56-1000

PODS®Technology

PODS®proteinsaremadeusinganinsectcellexpressionsysteminwhichtheactiveproteinisco-expressedalongsidepolyhedrincarrierprotein.Polyhedrinformsmicrocrystalswithininsectcellswhichspecificallycapturetheactiveproteintoformaco-crystalcomplex.Theactiveproteiniscapturedinitsnascent,nativelyfoldedformwithlimitedscopeforproteolyticdegradation.Consequently,excellentlevelsofbioactivityareobserved.ThePODS®co-crystalsprovideasustainedreleasemechanismandcanbeusedtofunctionalizesurfaces.Forfurtherdetails,pleaserefertothePODS®Technologypage.

ProductDescription

Theproductcontainsthepolyhedrinproteinco-crystalizedwithHumanBMP-4.BoneMorphogenicProtein4(BMP-4)isamemberoftheBoneMorphogeneticProtein(BMP)family.Theseproteinsaresynthesizedaslargeprecursormoleculeswhicharecleavedbyproteolyticenzymes.BMPproteinsstimulatetheproductionofbonematrixproteinsandosteoclastsproliferation,andcanbefoundontissuesrelatedwithboneorcartilagegrowth.

UsageRecommendation

PODS®co-crystalsprovideadepotofproteinswhicharesteadilysecreted.Ithasbeenestimatedthatthebiologicalactivityof50millionPODS®co-crystalsgeneratesthesamepeakdoseas3.3μgofstandardrecombinantprotein.However,at5daysfollowingthestartofseedingthePODS®co-crystals,therearemorethan50%ofthesepeaklevelsstillpresentintheculturesystem.Ultimately,theamountofPODS®co-crystalsthatisoptimalforaparticularexperimentshouldbedeterminedempirically.Basedonpreviousdata,wesuggestusing50millionPODS®co-crystalsinplaceof3.3μgofstandardgrowthfactorasastartingpoint."

Tocontrolforcross-reactivitywithcellsorasanegativecontrol,werecommendusingPODS®growthfactorsalongsidePODS®Emptycrystals,asthelatterdonotcontainorreleasecargoprotein.

AASequence

MADVAGTSNRDFRGREQRLFNSEQYNYNNSKNSRPSTSLYKKAGSIPGNRMLMVVLLCQVLLGGASHASLIPETGKKKVAEIQGHAGGRRSGQSHELLRDFEATLLQMFGLRRRPQPSKSAVIPDYMRDLYRLQSGEEEEEQIHSTGLEYPERPASRANTVRSFHHEEHLENIPGTSENSAFRFLFNLSSIPENEAISSAELRLFREQVDQGPDWERGFHRINIYEVMKPPAEVVPGHLITRLLDTRLVHHNVTRWETFDVSPAVLRWTREKQPNYGLAIEVTHLHQTRTHQGQHVRISRSLPQGSGNWAQLRPLLVTFGHDGRGHALTRRRRAKRSPKHHSQRARKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR

AlternativeNames

Bonemorphogeneticprotein4,bonemorphogeneticprotein4A,BMP-4A,BMP4,BMP4A

ResearchUseOnly

PODS®LimitedUseLabelLicense.Alicenseappliesforthisproduct,pleaseseeProductUserGuidebelowfordetails.

References
Documents
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ProductDetails
Length	452aa
MolecularWeight	103kDa
Structure	Dimer
Source	Spodopterafrugiperda(Sf9)cellculture
AccessionNumber	AAC72278.1
EndotoxinLevel	<0.06EU/mlasmeasuredbygelclotLALassay
Formulation	PODS®werelyophilizedfromavolatilesolution
Reconstitution	PODS®co-crystalsmaybereconstitutedat200millionco-crystals/mlinwater.20%glucosehasabuoyantdensityclosertoPODS®co-crystalsandcanbeusefulforaliquoting. PODS®co-crystalsarehighlystablewhenstoredinaqueoussolution(pHrange6-8).
StABIlityandStorage	Uponreceipt,storeat4°C.PODS®co-crystalsarestableforatleast1yearwhendryand6monthswhenresUSPended.

References

FasséliCoulibaly,ElaineChiu,KeikoIkeda,SaschaGutmann,PeterW.Haebel,ClemensSchulze-Briese,HajimeMori,andPeterMetcalf.Themolecularorganizationofcypoviruspolyhedra.(2007)Nature.446:97-101.

ReyFA.Virology:Holedupinanaturalcrystal.(2007)Nature.446:35-37.

MoriH.ImmobilizationofBioactiveGrowthFactorsintoCubicProteinousMicrocrystals(CypovirusPolyhedra)andControlofCellProliferationandDifferentiation.(2010)NSTI-Nanotech.3:222-225.

SatoshiAbe,HiroshiIjiri,HashiruNegishi,HiroyukiYamanaka,KatsuhitoSasaki,KunioHirata,HajimeMori,andTakafumiUeno.DesignofEnzyme-EncapsulatedProteinContainersbyIn-VivoCrystalEngineering.(2015)AdvancedMaterials.27(48):7951-7956.

Documents

PPH56Datasheet.pdfPODSUserGuide.pdfPODSMSDS.pdf

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Cellgs/PODS® Human BMP-4/PODS<sup>&reg;</sup> Human BMP-4, 1 billion/PPH56-1000