重组人铁蛋白重链/FTH1 Human,Ferritin heavy chain, Cell proliferation
重组人铁蛋白重链/FTH1Human,Ferritinheavychain,Cellproliferation-inducinggene15protein,FTH1,FHC,FTH,PLIF,FTHL6,PIG15,MGC104426.SYNONYMS
Ferritinheavychain,Cellproliferation-inducinggene15protein,FTH1,FHC,FTH,PLIF,FTHL6,PIG15,MGC104426.
INTRODUCTION
Ferritinisafairlylarge,iron-storageheteropolymericproteincomposedof2subunittypes,lightFerritin&heavyFerritinpolypeptides,whichisexpressedinmostkindsofcellsandco-assembleindifferentproportioninatissue-specificmanner.Ferritiniscomposedof24self-assembledpolypeptidesubunitsoftheheavyandlightferritinchainsandischaracterizedbythecapacitytoremoveFe(II)fromsolutioninthepresenceofoxygen. Ferritinlightpolypeptideproteinisthemainintracellularironstorageproteininprokaryotesandeukaryotes.Variationinferritinsubunitcompositioninfluencetheratesofironuptakeandreleaseinvarioustissues.Akeyfunctionofferritinisthestorageofironinasolubleandnontoxicstate.Defectsinthislightchainferritingeneareassociatedwithseveralneurodegenerativediseasesandhyperferritanemia-cataractsyndrome.
Ferritinstoresironinasoluble,nontoxic,readilyaccessibleform.Ferritinisneededforironhomeostasis.Ironistakenupintheferrousformanddepositedasferrichydroxidesafterithasbeenoxidized.
DESCRIPTION
FTH1HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining183aminoacidsandhavingamolecularmassof21kDa.
SOURCE
EscherichiaColi.
PHYSICALAPPEARANCE
SterileFilteredcolorlesssolution.
FORMULATION
TheFTH1proteinsolutioncontains20mMTris-HClpH-7.5,1mMDTTand10%glycerol.
STABILITY
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime. Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
AMINOACIDSEQUENCE
MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES.
PURITY
Greaterthan95.0%asdeterminedbySDS-PAGE.
USAGE
ProSpec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
