TheTruSeqSyntheticLong-ReadDNALibraryPrepKitisahighlyaccurate,end-to-endsequencingsolutionthatcanbeusedforgenomeassemblyorgenomephasing.Dependingontheanalysisoptionyouselect,youcanuseitto:
TheTruSeqSyntheticLong-ReadDNALibraryPrepandTruSeqSyntheticLong-ReadDNABarcodeKitsaredesignedforpreparingDNAlibrariestogeneratesyntheticallylongreads.ThelibraryprepkitcombinesTruSeqandNexterachemistrieswithsyntheticlong-readtechnologytoprepareDNAlibraries.Theaccompanyingbarcodekitincludes384indexesforlabelingthesamplesineachwell.Theseindexesarethenusedaftersequencingtoconstructsyntheticallylongfragmentsforlong-readassemblyandphasinganalysis.
Aftersequencing,push-buttonanalysisinBaseSpaceSequenceHubsimplifiesassemblyoflongreads.DatacanbetransferredfromanIlluminasequencinginstrumenttotheBaseSpaceSequenceHubcloudinstantly.
TheTruSeqLong-ReadAssemblyAppconstructslong,syntheticreadsfromshortersequencingreadsforaccurategenomeassemblyandgenomefinishing.TruSeqsyntheticlong-readtechnologyallowsyoutousethesamefamiliarplatformwithanewapplication.
TheTruSeqPhasingAnalysisAppcanbeusedtoperformwholehumangenomephasing,identifyinghaplotypeinformationandco-inheritedalleles,andphasingdenovomutations.Byconstructingsyntheticallylongfragmentsfromshortersequencingreads,thismethodprovidesmorecomprehensiveandaccuratephasingcomparedtoconventionaltriostudiesorstatisticalinference.
AssayTime | 3days |
Hands-OnTime | 6hours |
SpeciesCategory | AnySpecies |
SystemCompatibility | HiSeq2000,HiSeq3000,HiSeq2500,HiSeq4000 |
Method | Whole-GenomeSequencing,DeNovoSequencing,Long-ReadSequencing |
Technology | Sequencing |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn