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HansaBioMed/Immunoplate for Tumor-derived Exosome capture and enrichment from human plasma - Covalent coating (5 white p

ImmunoplatesforTumor-derived Exosomecaptureandenrichmentfromhumanplasma.

 

Applications:

Proteomicanalyses

Exosomesincorporateawiderangeofmembranesandcytosolicproteinsinvolvedinmanycellularfunctionsandreflectingboththeirendosomaloriginandaproteinfingerprintuniqueforparentcelltypeandcondition.HBMImmunoplatesareanusefulplatformforExosomecapturefromdifferentbiologicalsourcesandallowquantificationthroughELISAtechniqueofproteinexosomalMarkers.

WorkwithexosomalRNA

ExosomesshuttleRNAmolecules(mRNAsandmiRNAs),bothclassesprovedtobefunctionalintargetcell,beingtranslatedormodulatinggenesilencing,respectively.Overall,exosomemolecularcompositiondistinguishesrestingfromactivated,healthyfromdiseasedcells.

RNAextractioncouldbeperformedaftercapturingexosomesontheplate.RecommendedforRNAextraction:HansABIoMedExosomeTotalRNAExtractionKit.

Totalexosomequantification

ImmunoplatesarepartoftheReadytoUseKitforcaptureandquantification.Thekitcontainsallthenecessarycomponentsexosomequantification.

Relatedproducts:

Exosomestandards

RNAextractionkits

Quantificationkits

Product

White 5standard96-wellformatpre-coatedplates

Sampletype

Humanplasma

Exosomecapiture

EnrichmentinTumor-derivedexosome

Startingmaterial

Upto100µLofBIOLOGicalsamplecanbeloadedperwell.

Unfractionatedhumanplasmaosamplecanbedirectlyusedforcapture.

Concentratedurinesamplesarerecommendedpriorcaptureaccordingtooursuggestedprotocol.

Productoverview

Ourmultiwellplatesarestandard96-wellformatwhereassayscanbeconductedassingletonsand/ormultiplewells.ThisenablesoptimizationofawiderangeofsandwichELISAassaysorotherdownstreamapplications,suchasRNAextractionandproteomicanalyses.Transparent,whiteandblackplatesareavailabledependingonthedownstreamdetectionapproach(colorimetric,luminometricandfluorimetric).

Antibodyusedforcoating

Polyclonalrabbitantibody

Coating

Covalent

Packaging

Theplateispackagedinanopaquealuminumpouchwhichcompliestofoodandpharmaceuticalregulations.

Easytoopenandre-sealablebyzip-lockfeature.

Shipmentandstorage

Immunoplatesareshippedat4°Candstorageconditionsasrecommendedintheproductinsert.

Expirationdate

Unopened:2yearstoredat4°C

Opened:1monthstoredat4°C