HansaBioMed/Lipid Peroxidation (MDA) Assay Kit (100 reactions)/HBMK739100/1 Ea

Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit.

Sensitive, Colorimetric & Fluorometric Assay

Kit Summary:

• Detection method-Absorbance (532 nm) or Fluorescence (Ex/Em 532/553 nm)

• Sample type- Cell and Tissue culture supernatants, plasma and other biological fluids (optimized by end user).

• Species reactivity- All

• Applications- The kit detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.

Features & Benefits:

• Simple procedure

• Fast and convenient

• Sensitive assay for measuring lipid peroxidation (LPA) in a wide range of samples.

Kit Components:

• MDA Lysis Buffer

• Phosphotungstic Acid Solution

• BHT (100X)

• TBA

• MDA Standard (4.17M)

Description:

Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. HansaBioMed Lipid Peroxidation Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (λ = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.

Storage Conditions: -20°C.

Shipping Conditions: gel pack.

USAGE: For Research Use Only! Not For Use in Humans.