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HansaBioMed/Cytochrome c Releasing Apoptosis Assay Kit (100 reactions)/HBM-K257-100/1 Ea

CytochromecApoptosisAssayKit.

 

KitSummary:

•Detectionmethod-Westernblotting

•Sampletype-CellsandTissues

•Speciesreactivity-Mammalian

•Applications-Effectivemeansfordetectingcytochromectranslocationfrommitochondriaintocytosolduringapoptosis.Thekitprovidesaneasy-to-useprocedureandreagentsforseparatingamitochondria-enrichedfractionfromcytosol.

 

Features&Benefits:

•Simpleprocedure;takes~3hours

•Fastandconvenient

•CytochromecreleasingfrommitochondriaintocytosolisthendeterminedbyWesternblottingusingthecytochromecantibodyprovidedinthekit.Theprocedureissimpleandstraightforward.Noultracentrifugationisrequired.

 

KitComponents:

•MitochondriaExtractionBuffer

•5XCytosolExtractionBuffer

•DTT(1M)

•500XProteaseInhibitorCocktail

•Anti-CytochromecmousemAb(0.2mg/ml).

 

Description:

Cytochromecplaysanimportantroleinapoptosis.Theproteinislocatedinthespacebetweentheinnerandoutermitochondrialmembranes.AnapoptoticstimulustriggersthereleaseofcytochromecfromthemitochondriaintocytosolwhereitbindstoApaf-1.Thecytochromec/Apaf-1complexactivatescaspase-9,whichthenactivatescaspase-3andotherdownstreamcaspases.CytochromecReleasingApoptosisAssayKitprovidesaneffectivemeansfordetectingcytochromectranslocationfrommitochondriaintocytosolduringapoptosis.Thekitprovidesuniqueformulationsofreagentstoisolateahighlyenrichedmitochondriafractionfromcytosol.Theprocedureissosimpleandeasytoperform,noultracentrifugationisrequiredandnotoxicchemicalsareinvolved.CytochromecreleasingfrommitochondriaintocytosolisthendeterminedbyWesternblottingusingthecytochromecantibodyprovidedinthekit.

 

StorageConditions:-20°C.

 

ShippingConditions:gelpack.

 

USAGE:ForResearchUseOnly!NotForUseinHumans.

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