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HansaBioMed/DNA Damage Quantification Kit (25 reactions)/HBM-K253-25/1 Ea

DNADamageQuantificationColorimetricKit.

 

KitSummary:

•Detectionmethod-Absorbance(450and650nm)

•Speciesreactivity-Mammalian

•Applications-KitprovidesallnecessaryreagentsforconvenientdeterminationofDNAdamage(abasicsites)insampleDNA.

 

SampleType:

PurifieddamagedDNA

 

Features&Benefits:

Kitprovidesallnecessaryreagentsfor96-wellplateformat.

 

KitComponents:

•ARPSolution

•TEBuffer

•GlycogenSolution

•0ARP-DNAStandard

•40ARP-DNAStandard

•DNABindingSolution

•HRP-Streptavidin

•10XWashBuffer

•HRPDeveloper

•96-wellMicroplate(8x12strips).

 

Description:

Apurinic/apyrimidinic(AP)sitesareoneofthemajortypesofDNAlesionsformedduringthecourseofbaseexcisionandrepairofoxidized,deaminatedoralkylatedbases.Ithasbeenestimatedthatabout2x105baselesionsaregeneratedpercellperday.ThelevelofAPsitesincellscanbeagoodindicatorofDNAlesionandrepairagainstchemicaldamageandcellaging.TheDNADamageQuantificationKitutilizestheARP(AldehydeReactiveProbe)reagentthatreactsspecificallywithanaldehydegroupwhichistheopenringformoftheAPsites.AftertreatingDNAcontainingAPsiteswithARPreagents,APsitesaretaggedwithbiotinresidues,whichcanbequantifiedusingavidin-biotinassayfollowedbyacolorimetricdetection.ThekitprovidesthenecessaryreagentsforconvenientdeterminationofabasicsitesinpurifiedDNAsamplein96-wellplateformat.

 

StorageConditions:+4°C.

 

ShippingConditions:gelpack.

 

USAGE:ForResearchUseOnly!NotForUseinHumans.

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