HansaBioMed/Glutathione Reductase Assay Kit (200 rections)/HBMK761200/1 Ea

Glutathione Reductase Activity Colorimetric Assay Kit.

Highly Sensitive Assay, HTS

Kit Summary:

• Detection method- Absorbance (405 nm)

• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, erythrocyte lysates, as well as many other biological fluids etc.

• Species reactivity- Mammalian

• Application- The assay can detect 0.1 – 40 mU/ml GR in various samples.

Features & Benefits:

• Simple procedure; takes ~ 30-40 minutes

• Fast and convenient

Kit Components:

• GR Assay Buffer

• 3% H₂O₂

• Catalase

• TNB Standard (5 mM)

• DTNB

• NADPH-GNERAT™

• GSSG

• GR Positive Control (10 mU)

Description:

Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. HansaBioMed’s Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5′-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1 – 40 mU/ml GR in various samples.

Storage Conditions: -20°C.

Shipping Conditions: gel pack.

USAGE: For Research Use Only! Not For Use in Humans.