Beclin-1是自噬的关键正调控因子，是自噬体形成的必要条件，Novus的Tat-D11将Beclin-1上发挥重要作用的11个氨基酸与一种细胞渗透性肽相融合，有效诱导细胞自噬。原理如下图所示，GAPR-1与Beclin-1结合后抑制自噬形成，而Tat-D11的存在能够让Beclin-1解离下来，从而诱导自噬形成。

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Figure1.Tat-D11andTat-L11aremorepotentautophagyinducersthanTat-Beclin1.HeLaGFP-LC3BweretreatedwithTat-D11,Tat-L11,Tat-L11SorTat-Beclinfor1.5hrs,and(A)thenumberofautophagosomeswereassessedbyfluorescentmicroscopyand(B)thenumberofGFP+/LC3B+spotswerequantified

Figure2.Dose-responseinductionofGFP-LC3BinHelacellsbyTat-D11.InsetfigureshowsthattheTat-D11isamorepotentinducerofautophagythantheTat-Beclin-1peptide.

Figure3InductionofLC3Banddownregulationofp62inHelacellsbyTat-D11andTat-L11.

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​​​​​​​联科生物代理截止时间：2017年6月30日