IGF-Ireceptorisadisulfide-linkedheterotetramerictransmembraneproteinconsistingoftwoalphaandtwobetasubunits.BoththealphaandbetasubunitsareencodedwithinasinglereceptorprecursorCDNA.Theproreceptorpolypeptideisproteolyticallycleavedanddisulfide-linkedtoyieldthematureheterotetramericreceptor.ThealphasubunitofIGF-Ireceptorisextracellularwhilethebetasubunithasanextracellulardomain,atransmembranedomainandacytoplasmictyrosinekinasedomain.TheIGF-Ireceptorishighlyexpressedinallcelltypesandtissues.
Images:(1)LysateswerepreparedfromNTERA-2humanembryonalcarcinomacells,SK-MEL-28humanmalignantmelanomacells,andG-361humanmalignantmelanomacellsinnon-reducingsamplebuffer.ThelysateswereresolvedbySDS-PAGEandtransferredtoanImmobilon-Pmembrane.Theblotwasdevelopedwith1μg/mLmonoclonalanti-humanIGF-IRantibodyandWesternGloChemiluminescentDetectionSubstrate.(2) IGF-IR(atadilutionof25µg/mL) stainingof paraffin-embeddedhumanskintissue. Tissue stainedwith anti-mouseHRP-DAB(Brown)andcounterstainedwithhaematoxylin(blue).
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn