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Prospec/GFRA1 Human/CYT-1026/2µg

Cataloguenumber

CYT-1026

Synonyms

GDNFreceptoralpha-1,GDNFR-alpha-1,GFRalpha-1,RETligand1,TGF-beta-relatedneurotrophicfactorreceptor1,GDNFRA1,RET1L2,RETL1,GlialCellLineDerivedNeurotrophicFactorReceptorAlpha,TRNR1,GPILinkedAnchorProtein,PI-LinkedCell-Surface.

Introduction

GDNFfamilyreceptoralpha-1(GFRA1)belongstotheGDNFreceptorfamily.GFRA1isaglycosyl-phosphatidylinositol(GPI)-linkedcellsurfacereceptorforbothGlialcellline-derivedgrowthfactor(GDNF),neurturin(NTN),andmediatesactivationoftheRETtyrosinekinasereceptor.TheGFRA1proteinisapotentsurvivalfactorforcentralandperipheralneurons,andisvitalforthedevelopmentofkidneysandtheentericnervoussystem.

Description

GFRA1HumanRecombinantproducedinHEK293cellsisasingle, glycosylatedpolypeptidechain(a.a25-423)containing409aminoacidsincludinga10a.aC-terminalHistag.Thetotalmolecularmassis46.0kDa(calculated). 

Source

HEK293cells.

PhysicalAppearance

FilteredWhitelyophilized(freeze-dried)powder.

Formulation

GFRA1filtered(0.4µm)andlyophilizedfrom0.5mg/mlinphosphatebufferedsalinepH7.5containing5%(w/v)trehalose.

Solubility

Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.GFRA1isnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.

StABIlity

Storelyophilizedproteinat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeaftertwoweeksat4°C.

Purity

Greaterthan95.0%asdeterminedbySDS-PAGE.

Aminoacidsequence

DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLASGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFISVEHIPKGNNCLDAAKACNLDDICKKYRSAYITPCTTSVSNDVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSSCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPAFPVQTTTATTTTALRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGNTHLCISNGNYEKEGLGASHHHHHHHHHH.

Usage

Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.

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