Thiskitisidealforuserswhowantaconvenientandsensitiveassay.TheassayisdesignedfortheenumerationofcellssecretinghumanIL-4.ThekitincludesELISpotplatespre-coatedwithmonoclonalantibody,biotinylateddetectionantibody,Streptavidin-ALPandready-to-useBCIP/NBT-plussubstrate.ApolyclonalactivatorforhumanTcells(mAbCD3-2)issuppliedasapositivecontrolforcytokinesecretion.
Pre-coatedplatesreduceassaytimeandminimizevariABIlity.
HumanIL-4ELISpotPLUSkitsarealsoavailablewithStreptavidin-HRPandTMBsubstrate.SeeRelatedproducts.
Themonoclonalantibodiesinthiskitcross-reactwithIL-4fromnon-humanprimates.SeetheHuman/NHPcross-reactivitytable.
IL-4
Interleukin-4(IL-4,IL4)isproducedbyactivatedTcells,especiallydifferentiatedCD4+TH2-typecells,andstimulatedmastcellsandbasophils.ThemostimportanteffectsofIL-4aretheregulationofhelperTcelldifferentiationtotheTH2typeandtheregulationofIgEandIgG4productionbyBcells.
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| Size: | 10plates | |
| Otherformats: | ClicktoviewallHuman/NHPIL-4products | |
| Minimizeworkloadwiththeone-stepdetectionELISpotPROkit | ||
IhavecomparedMabtechELISpotassaytoELISpotassayswerunatourcompanyonregularbasis"head-to-head"usingidenticalsamples.Mabtech"skitsprovedtobesuperiortoourprotocol,hadsignificantlylowerbackgroundandprovidedbetterandhigherdetectionofspots.
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