Thiskitisidealforuserswhowantaconvenientandsensitiveassay.TheassayisdesignedfortheenumerationofcellssecretinghumanIL-21.ThekitincludesELISpotplatespre-coatedwithmonoclonalantibody,biotinylateddetectionantibody,Streptavidin-HRPandready-to-useTMBsubstrate.
Pre-coatedplatesreduceassaytimeandminimizevariABIlity.
HumanIL-21ELISpotPLUSkitsarealsoavailablewithStreptavidin-ALPandBCIP/NBT-plussubstrate.SeeRelatedproducts.
Themonoclonalantibodiesinthiskitcross-reactwithIL-21fromnon-humanprimates.SeetheHuman/NHPcross-reactivitytable.
IL-21
Interleukin-21(IL-21,IL21)isprimarilysecretedbyCD4+Tcells(inparticularfollicularTcellsandTh17)andisanimportantregulatorofimmuneresponses;forTh17cells,IL-21isanautocrineregulatorsustainingTh17development.IL-21alsoregulatesthedevelopmentofotherTcellsaswellasBcells,NKcellsanddendriticcells.AreasofparticularinterestforresearchonIL-21includeautoimmuneandinflammatorydiseases,cancerresearchbutalsobasicstudiesone.g.BcellsandTcells,inparticularTh17cells.
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| Size: | 2plates | |
| Otherformats: | ClicktoviewallHuman/NHPIL-21products | |
IhavecomparedMabtechELISpotassaytoELISpotassayswerunatourcompanyonregularbasis"head-to-head"usingidenticalsamples.Mabtech"skitsprovedtobesuperiortoourprotocol,hadsignificantlylowerbackgroundandprovidedbetterandhigherdetectionofspots.
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