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TheFluoroSpotassayisaninventivedevelopmentoftheELISpotassayandusesfluorescentdetectioninsteadofcolorimetric.Thisallowsforsimultaneousdetectionofseveralanalytes,whichisamajoradvantage.ThehumanIL-6/IL-12/-23(p40)FluoroSpotkitenablesconvenientenumerationofcellssecretingeitheroneorbothanalytes.Thekitcontainslowfluorescentplates,matchedquantitiesofmonoclonalcaptureanddetectionantibodies,secondaryreagentsconjugatedtofluorophoresandafluorescenceenhancer.LPSisincluded.
Theassayisrecommendedfortheanalysisofmacrophages,monocytesanddendriticcells.ThesekitshavenotbeenevaluatedforT-cells.
Foranalysis,werecommendtheuseofanautomatedFluoroSpotreaderequippedwithfiltersforFITC(excitation490nm/emission510nm)andCy3(excitation550nm/emission570nm).Filtersshouldhavehighspecificitytoavoidbleed-throughartifacts.Doublesecretingcellsareidentifiedbyacomputerizedoverlay.
IL-12/-23(p40)
Interleukin-12(IL-12,IL12)is,whenBIOLOGicallyactive,aheterodimer(p70)consistingoftwocovalentlylinkedsubunits,p35andp40.IL-12isproducedbyantigen-presentingcells,specificallydendriticcellsandmacrophages.IL-12isaproinflammatorycytokinethatpromotesTh1-typeresponsesbyinducingIFN-γproductionandenhancingproliferationandcytotoxicityofNKandTcells.Interleukin-23(IL-23)isadisulfide-linkedheterodimerwhichconsistsofauniquep19subunitandthep40subunitofIL-12.IL-23isaproinflammatorycytokine,whichisproducedbymacrophagesanddendriticcellsuponinfectionswithcertainpathogens.TheIL-23receptorisexpressedonTh17cellsandIL-23isresponsIBLeforTh17expansionandstABIlization.SincebothIL-12andIL-23heterodimerscomprisethep40subunit,captureimmunoassaysbasedonantibodiestop40willdetectbothcytokines.Suchassayscanalsodetectp40monomersanddimers.
IL-6
Interleukin6(IL-6,IL6)isapleiotropiccytokinethatisproducedbymanydifferentcelltypesandplaysaroleinawiderangeoffunctions,suchasimmuneresponses,acute-phasereactions,andhematopoiesis.AmongotherthingsitaugmentsantibodyproductionfromactivatedBcellsinvitro.
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| Size: | 10plates |
| Otherformats: | ClicktoviewallHumanIL-12/-23(p40),IL-6products |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn