Type
Recombinant
Description
TRAILisacytotoxicprotein,whichactivatesrapidapoptosisintumorcells,butnotinnormalcells.TRAIL-inducedapoptosisisachievedthroughbindingtotwodeath-signalingreceptors,DR4andDR5.ThesereceptorsbelongtotheTNFRsuperfamilyoftransmembraneproteins,andcontainacytoplasmic“deathdomain”,whichactivatesthecell’s apoptoticmachinery.RecombinantMurineTRAILisa174 aminoacidpolypeptide(20.0 kDa),consistingoftheTNF-homologousportionoftheextracellulardomainofthefulllengthTRAILprotein.
AminoAcidSequence
MRGGRPQKVAAHITGITRRSNSALIPISKDGKTLGQKIESWESSRKGHSFLNHVLFRNGELVIEQEGLYYIYSQTYFRFQEAEDASKMVSKDKVRTKQLVQYIYKYTSYPDPIVLMKSARNSCWSRDAEYGLYSIYQGGLFELKKNDRIFVSVTNEHLMDLDQEASFFGAFLIN
Source
E.coli
Purity
95%
BIOLOGicalActivity
Assay#1:Determinedbythedose-dependentstimulationofMIP-2 productionbymousespleencellsusingaconcentrationrangeof10–100ng/ml.Assay#2:MeasuredbyitsABIlitytoinduceapoptosisinLN-18 cells(humanglioblastomacells).TheexpectedED50forthiseffectis40.0–60.0 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C