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| Synonyms | MonoclonalBTRCantibody,Anti-BTRCantibody,Beta-transducinrepeatcontainingantibody,FBXW1Aantibody,bTrCPantibody,bTrCP1antibody,E3RSIkappaBantibody,pIkappaBalpha-E3receptorsubunitantibody,Fwd1antibody,FBW1Aantibody |
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| CrossReactivity | Human |
| Applications | WB |
| Immunogen | BTRCantibodywasraisedinmouseusingahumanfragmentcorrespondingtoaminoacids52-354ofhumanBTRC(NP_378663)producedinE.coliastheimmunogen. |
| Host | Mouse |
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| Clone | 1D4 |
| Isotype | IgG1 |
| MethodofPurification | BTRCantibodywaspurifiedbyaffinitychromatography. |
| Form&Buffer | SuppliedinPBSbuffer,pH7.3,containing1%BSA,50%glyceroland0.02%sodiumazide. |
| Concentration | 500ug-1mg/ml |
| Storage | Storeat4degCforshorttermstorage.Aliquotandstoreat-20degCforlongtermstorage.Avoidrepeatedfreeze/thawcycles. |
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| UsageRecommendations | WB:1:500 |
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| BiologicalSignificance | BTRCisamemberoftheF-boxproteinfamilywhichischaracterizedbyanapproximately40aminoacidmotif,theF-box.TheF-boxproteinsconstituteoneofthefoursubunitsofubiquitinproteinligasecomplexcalledSCFs(SKP1-cullin-F-box),whichfunctioninphosphorylation-dependentubiquitination.TheF-boxproteinsaredividedinto3classes:FbwscontainingWD-40domains,Fblscontainingleucine-richrepeats,andFbxscontainingeitherdifferentprotein-proteininteractionmodulesornorecognizablemotifs.TheproteinencodedbythisgenebelongstotheFbwsclass;inadditiontoanF-box,thisproteincontainsmultipleWD-40repeats.ThisproteinishomologoustoXenopusbTrCP1,yeastMet30,NeurosporaScon2andDrosophilaSlimbproteins.ItinteractswithHIV-1VpuandconnectsCD4totheproteolyticmachinery. |
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