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| Synonyms | MonoclonalPRKAR2Aantibody,Anti-PRKAR2Aantibody,ProteinkinasecAMP-dependentregulatorytypeIIalphaantibody,PKR2antibody,PRKAR2antibody,MGC3606antibody,cAMP-dependentproteinkinasetypeII-alpharegulatorysubunitantibody,PRKAR2A,PRKA2Ra,PRKA-2Ra,PRKA2Raantibody,PRKA-2Raantibody |
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| CrossReactivity | Human |
| Applications | IF,IHC,WB |
| Immunogen | PRKAR2AantibodywasraisedinmouseusingafulllengthrecombinantproteinofhumanPRKAR2A(NP_004148)producedinHEK293Tcells,astheimmunogen. |
| Host | Mouse |
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| Clone | 4A3 |
| Isotype | IgG1 |
| MethodofPurification | PRKAR2Aantibodywaspurifiedbyaffinitychromatography. |
| Form&Buffer | SuppliedinPBSbuffer,pH7.3,containing1%BSA,50%glyceroland0.02%sodiumazide. |
| Concentration | 500ug-1mg/ml |
| Storage | Storeat4degCforshorttermstorage.Aliquotandstoreat-20degCforlongtermstorage.Avoidrepeatedfreeze/thawcycles. |
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| UsageRecommendations | IF:1:100,IHC:1:50,WB:1:2000 |
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| BiologicalSignificance | cAMPisasignalingmoleculeimportantforavarietyofcellularfunctions.cAMPexertsitseffectsbyactivatingthecAMP-dependentproteinkinase,whichtransducesthesignalthroughphosphorylationofdifferenttargetproteins.Theinactivekinaseholoenzymeisatetramercomposedoftworegulatoryandtwocatalyticsubunits.cAMPcausesthedissociationoftheinactiveholoenzymeintoadimerofregulatorysubunitsboundtofourcAMPandtwofreemonomericcatalyticsubunits.Fourdifferentregulatorysubunitsandthreecatalyticsubunitshavebeenidentifiedinhumans.Theproteinencodedbythisgeneisoneoftheregulatorysubunits.Thissubunitcanbephosphorylatedbytheactivatedcatalyticsubunit.ItmayinteractwithvariousA-kinaseanchoringproteinsanddeterminethesubcellularlocalizationofcAMP-dependentproteinkinase. |
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