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NSJ/ABCA1 Antibody (R31847)/ R31847/ 100 ug

Formulation:

0.5mg/mlifreconstitutedwith0.2mlsterileDIwater

Data:

AvailABIlity1-3businessdays
SpeciesReactivityHuman,Mouse,Rat
FormatAntigenaffinitypurified
ClonalityPolyclonal(rabbitorigin)
IsotypeRabbitIgG
PurityAntigenaffinity
BufferLyophilizedfrom1XPBSwith2.5%BSAand0.025%sodiumazide
UniProtO95477
ApplicationsWesternblot:0.1-0.5ug/ml
LimitationsThisABCA1antibodyisavailableforresearchuseonly.

Description:

ABCA1(ATP-bindingcassette,sub-familyA(ABC1),member1),alsoknownasABC1,thecholesteroleffluxregulatoryprotein(CERP)isaproteinwhichinhumansisencodedbytheABCA1gene.Themembrane-associatedproteinencodedbythisgeneisamemberofthesuperfamilyofATP-bindingcassette(ABC)transporters.ABCproteinstransportvariousmoleculesacrossextra-andintracellularmembranescontain2,261aminoacids.Dotblotanalysisof50tissuesrevealedubiquitousexpressionofABCA1mRNA,withhighestexpressioninplacenta,liver,lung,adrenalglands,andallfetaltissuesexamined,andlowestexpressioninkidney,pancreas,pituitary,mammarygland,andbonemarrow.ThisproteinisamemberoftheABCAsubfamily.MembersoftheABCAsubfamilycomprisetheonlymajorABCsubfamilyfoundexclusivelyinmulticellulareukaryotes.Withcholesterolasitssubstrate,thisproteinfunctionsasacholesteroleffluxpumpinthecellularlipidremovalpathway.UsinghumanABCA1expressedinthemembranefractionofsf9insectcells,Szakacsetal.foundspecific,Mg(2+)-dependentATPbindingandlowbasalATPaseactivity.AdditionofpotentiallipidsubstratesorlipidacceptorsdidnotmodifytheATPaseactivityornucleotideocclusionbyABCA1.Szakacsetal.speculatedthatABCA1maybearegulatoryproteinormayrequireotherproteinpartnersforfullactivation.

ApplicationNotes:

OptimaldilutionoftheABCA1antibodyshouldbedeterminedbytheresearcher.

Immunogen:

AminoacidsKDLSLHKNQTVVDVAVLTSFLQDEKVKESYVofhumanABCA1wereusedastheimmunogenfortheABCA1antibody.

Storage:

Afterreconstitution,theABCA1antibodycanbestoredforuptoonemonthat4oC.Forlong-term,aliquotandstoreat-20oC.Avoidrepeatedfreezingandthawing.
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