Deprecated: Required parameter $cat_id follows optional parameter $type in /www/wwwroot/ebimall.com/systems/hong.php on line 2088

Deprecated: Required parameter $where follows optional parameter $tree_id in /www/wwwroot/ebimall.com/systems/hlb.php on line 3505
In Situ Cell Death Detection Kit, POD 过氧化物酶标记的原位细胞凋亡检测试剂盒188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
您好,欢迎您进入188进口试剂采购网网站! 服务热线:4000-520-616
蚂蚁淘商城 | 现货促销 | 科研狗 | 生物在线

In Situ Cell Death Detection Kit, POD 过氧化物酶标记的原位细胞凋亡检测试剂盒
InSituCellDeathDetectionKit,POD

Kitforthe detectionandquantificationofapoptoticcelldeathonasingle-celllevelbylightmicroscopyinimmunocyto-andimmunohistochemistry

Application
Qualitativedetectionofapoptosisatthesingle-celllevelbylightmicroscopy.
Benefits
  • Sensitive:Themaximumintensityoflabeling(cellstaining)ofapoptoticcellsishigherthanthenicktranslationmethod
  • Fast:Theuseoffluorescein-dUTPallowsanalysisofthesamplesdirectlyaftertheTUNELreaction,butbeforetheadditionofthesecondarydetectionsystem
  • Convenient:Thedirectlabelingprocedureusingfluorescein-dUTPallowsverificationoftheefficiencyoftheTUNELreactionduringtheassayprocedure
  • Accurate:Identificationofapoptosisatamolecularlevel(DNA-strandbreaks)andidentificationofcellsattheveryearlystagesofapoptosis
  • FlexIBLe:Nosubstrateincluded;providestheopportunitytoselectthestainingprocedureofchoice
ProductDescription
Samplematerial:Cytospinandcellsmearpreparations,adherentcellsgrownonslides,andfrozenandparaffin-embeddedtissuesections.
BackgroundInformation
WidelyusedmethodstodetermineapoptosisincludetheanalysisofthegenomicDNAbyagarose-gelelectrophoresisandDNAfragmentationassaysbasedon3H-thymidineand,alternatively,5-Bromo-2-deoxy-uridine.Themethodsinvolvetheseparationoffragmented,lowmolecular-weightDNAfromunfragmented,highmolecular-weightDNAinagivencellpopulation.Thus,thesemethodsdonotprovideinformationaboutthefateofanindividualcellinagivencellpopulationor,particularly,intissuesections.Alternatively,individualapoptoticcellsmaybemicroscopicallyrecognizedbecauseofthecharacteristicappearanceofnuclearchromatincondensationandfragmentation,butthismethodissubjectiveandlimitedtoarelativelynarrowtimewindowwhenthemorphologicalchangesareatamaximum.
ThehallmarkofapoptosisisDNAdegradation,whichinearlystagesisselectivetotheinternucleosomalDNAlinkerregions.TheDNAcleavagemayyielddouble-strandedandsingle-strandedDNAbreaks(nicks).Bothtypesofbreakscanbedetectedbylabelingthefree3-OHterminiwithmodifiednucleotides(e.g.,biotin-dUTP,DIG-dUTP,fluorescein-dUTP)inanenzymaticreaction.Theenzymeterminaldeoxynucleotidyltransferase(TdT)catalyzesthetemplate-independentpolymerizationofdeoxyribonucleotidestothe3-endofsingle-anddouble-strandedDNA.ThismethodhasalsobeentermedTUNEL(TdT-mediateddUTP-Xnickendlabeling).Alternatively,free3-OHgroupsmaybelabeledusingDNApolymerasesbythetemplate-dependentmechanismcallednicktranslation.However,theTUNELmethodisconsideredtobemoresensitiveandfaster.
Contents
  1. EnzymeSolution(TdT),5vials
  2. LabelSolution(fluorescein-dUTP),5vials
  3. ConverterPOD(anti-fluoresceinantibody-POD),ready-to-use
Principle
TheInSituCellDeathDetectionKit,POD isbasedonthedetectionofsingle-anddouble-strandedDNAbreaksthatoccurattheearlystagesofapoptosis.
ApoptoticcellsarefixedandpermeABIlized.Subsequently,thecellsareincubatedwiththeTUNELreactionmixturethatcontainsTdTandfluorescein-dUTP.Duringthisincubationperiod,TdTcatalyzestheadditionoffluorescein-dUTPatfree3-OHgroupsinsingle-anddouble-strandedDNA.Afterwashing,thelabelincorporatedatthedamagedsitesoftheDNAismarkedbyananti-fluoresceinantibodyconjugatedwiththereporterenzymeperoxidase.Afterwashingtoremoveunboundenzymeconjugate,thePODretainedintheimmunecomplexisvisualizedbyasubstratereaction.

Figure1:Testprinciple.
 

订购指南：以下产品部分现货供应

 

检测方法

产品名称

产品货号

规格

价格￥

FACSFluorescenceorlightmicroscopies

Annexin-V-FLUOS

11828681001

250T

5023

Annexin-V-Alexa568

03703126001

250T

5443

Annexin-V-Biotin

11828690001

250T

5023

Annexin-V-FLUOSStainingKit

11858777001

50T

1932

Annexin-V-FLUOSStainingKit

11988549001

250T

6233

ELISA

Caspase3ActivityAssay

12012952001

96T

6578

CellDeathDetectionELISAPLUS

11774425001　

96T

3814

  TUNEL

InSituCellDeathDetectionKit,Fluorescein

11684795910

50T

4662

InSituCellDeathDetectionKit,AP

11684809910

50T

5267

InSituCellDeathDetectionKit,POD

11684817910

50T

5267

InSituCellDeathDetectionKit,TMRred

12156792910

50T

3931

 

北京分公司

上海市徐汇区南丹东路18号12楼A座

北京朝阳区大屯路2号科华商务大厦613室

4006-111-883

010-64847623   010-64855926

sales@qcbio.com 

lwh@qcbio.com


新闻动态
行业前沿
技术文章
最新产品