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Avidity/Expresso Biotin Cloning & Expression System/Lucigen(orderdirectly)

CLICK HERE toaccessLucigen"swebsiteforordering Expresso®BiotinCloning&ExpressionSystemproducts,additionalinformationandprotocols.

The Expresso®BiotinCloning&ExpressionSystemsarenowavailablefromourbusinesscollaboratorLucigenCorporation. ThesesystemsofferyouthequickestroutetobiotinylatedproteinbycombiningtheAviTag™andEpressioneering™technologies.

LucigenhaspartneredwithAvidity,LLCtoleveragethepoweroftheAviTag™minimalbiotinylationmotifandLucigen"sSUMOsolubilityfusiontagtosolvethemostdifficultproblemsinproteinexpressionandpurification.TheincludedBiotinXCell™competentcellswillefficientlybiotinylateproteinbearingtheAviTagmotifinvivo,meaningyoucanskiptheinvitroandchemicalmethodsotherwiserequiredtoobtainbiotinylatedtargetprotein.TheSUMOtagwillproducelargeamountsofsolubleproteinthatcanbepreciselycleavedwithitscognateprotease.SinglecolumnpurificationofAviTagproteinsiscompletedinafewhoursusingthebiotin-streptavidininteraction,andoncolumncleavageoftheAviTag-SUMOfusionfollowedbynickelcolumnchromatographyoftheHIStaggedproteaseyields>95%pureprotein.

 

 

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