AntiHuman PGP 9.5 (UCHL1) (184), (affinity purified) (Rabbit IgG) Cedarlane

ImmunohistochemicalanalysisofParaffinEmbeddedSections

CL7755AP(Anti-HumanPGP9.5)staining(10µg/ml)ofahumancerebralcortexformalin-fixed,paraffin-embeddedtissuesection;seenat10x(left)and20x(right)magnification.Stainingonnormalbraincortexisnegative.Highgradetumor(glioma)showssharpnuclearandcytoplasmicstaininginmorethan70%ofthelesion.

CL7756AP(Anti-HumanPGP9.5)staining(10µg/ml)ofahumancerebralcortexformalin-fixed,paraffin-embeddedtissuesection;seenat20x(left)and40x(right)magnification.Stainingonnormalbraincortexisnegative.Highgradetumor(glioma)showssharpnuclearandcytoplasmicstaininginmorethan70%ofthelesion.

CL7756AP(Anti-HumanPGP9.5)staining(2µg/ml)ofahumancolonformalin-fixed,paraffin-embeddedtissuesection;seenat20x(left)and40x(right)magnification.Cytoplasmic/membranestainingofperipheralnerve/ganglionandstromalcellsareobserved.

CL7756AP(Anti-PGP9.5)staining(0.1µg/ml)ofratepidermistissuesectionsisolatedfromratpawusingthermolysin.ThetissuewasfrozeninM1embeddingmatrixandcutonacryostatto12µm.Itwasfixedina0.1MsolutionofPBScontaining0.2%(w/v)paraformaldehydeand0.75%(w/v)picricacid.Sectionswereimagedusingconfocalmicroscopy.IntraepithelialnervestainingofPGP9.5isobserved(green);tissuewascounterstainedwithDAPI(blue).ImageprovidedbyMichaelAndersonfromOklahomaStateUniversity,CenterforHealthSciences.PhDstudent.

Immunofluorescenceanalysis

Anti-PGP9.5(CL7755AP)stainingusinggoatanti-rabbitAlexa-488secondaryantibodyonAdultMouseHypothalamusCellLinemHypoA-2/12cells(CLU177)imagedunderconfocalmicroscopewiththe40Xoilobjective;nucleiwerecounterstainedwithDAPI(blue).

WesternBlotAnalysis

CL7755AP(0.1µg/ml)stainingofHumanCerebellum(A),MouseBrain(B)andRatBrain(C)lysates(35µgproteininRIPAbuffer).Primaryincubationwas1hour.Detectedbychemiluminescence

CL7756AP(0.01µg/ml)stainingofHumanCerebellum(A),MouseBrain(B)andRatBrain(C)lysates(35µgproteininRIPAbuffer).Primaryincubationwas1hour.Detectedbychemiluminescence.

Description

ProteinGeneProduct(PGP)isanabundantcytoplasmicneuronandneuroendocrine-cellspecificprotein.Inrecentyears,antibodiestothisproteinhavebecomeestablishedasMarkersforneuronsandcellsofthediffuseneuroendocrinesystem(DNES).PGP9.5appearstobelongtoafamilyofubiquitin-carboxyterminalhydrolaseisoenzymes,butwhileitshowsactivitytowardssyntheticubiquitinesters,theinvivosubstrate(s)ofPGP9.5remainunknown.MonoclonalandpolyclonalantibodiesagainstPGP9.5willstainneuronalcellbodiesandaxonsinthecentralandperipheralnervoussystems,smallnervefibersinnervetissue,neuroendocrinecellsinthepituitary,thyroidpancreasetc.,andalsotumorsoftheDNES.Antibodiestothisproteinmarkercanbeappliedinneuropathology,inexperimentalstudiesofinnervationsofperipheraltissuesandinstudiesofthedevelopmentandpathologyoftheDNES.ItisemphasizedthatantibodiestoPGPareparticularlysuitablefordetectingsmallnervefibersinperipheraltissues.