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北京乐博生物,ChromoTek中国授权代理,邀您免费申请GFP-Trap小样!
http://www.testrust.com 来源:北京乐博生物科技有限公司 时间:2014-05-26
活动申请:
1、联系 info@ChromoTek.com 邮箱,提交小样申请(货号:gta-2,规格50ul);
2、申请成功后把厂家回复的邮件转发到 lab_china@163.com 邮箱,随邮件发下姓名、科室电话、手机、单位、收货地址、发票抬头等信息;
One step一步法完成IP | Co-IP | ChIP,让您的免疫沉淀更简易,更便捷,更可靠,更专业!
GFP-Trap_A
产品描述: 与agarosebeads结合
particlesize~80 m
存储缓冲液:20%EtOH
储存方法:室温运输,收到后4℃储存一年。请勿冰冻。
Nano-Traps 值得信赖的科研工具
Chromotek的Nano-Trap 基于alpaca的单域抗体片段(VHHS)
小型
极端稳定
70℃仍保持稳定,functionalin2MNaClor0.5%SDS
高亲和力
解离常数范围在sub-nM内
高特异性
GFP-Trap 产品特点(高稳定性、高亲和力)
特异性结合绿色荧光蛋白的衍生物,如GFPS65T,YPF或EYFP,不与DsRed衍生蛋白结合。(见FAQs)
多功能且功能强大的GFP融合蛋白生化实验
缩短孵化时间(只需5-30min)
从细胞提取物或细胞器中定量分离融合蛋白及结合(暂时性)因子
超低的非特异性结合影响
无污染的普通抗体的轻链与重链
适用于染色质免疫沉淀(ChIP)
GFP-Trap 产品应用:
免疫沉淀IP/Co-IP
质谱分析
酶活测定
生物大分子相互作用/亲和力实验
染色质免疫沉淀(ChIP,ChromatinImmunoprecipitation)
Nano-Trapsreliableresearchreagents
NanoTrapsarebasedonrecombinantsingledomainantibodyfragments(VHHS)derivedfrom
alpacacoupledtomonovalentmatrices(agarosebeads,magneticparticlesormultiwellplates).
OurGFPTrap /RFPTrap arenovelhighqualitytoolsforthefast,reliableandefficientonestep
isolationoffluorescentfusionproteinsandtheirinteractingfactors.
Features
Robustandversatiletoolsforbiochemicalanalysesoffluorescentfusionproteins
Shortincubationtimes(5 30min)
Quantitativeisolationoffusionproteinsandtransientlyboundfactorsfromvariouscellextracts
andorganelles(fromallorganisms)
Lowunspecificbinding
Nounspecificbindingorcontaminationbyheavyandlightchainsofconventionalantibodies
GFP-Trap
OurGFP-Trap isahighqualityGFP-bindingproteincoupledtoamonovalentmatrix(agarosebeads,
magneticparticlesorin96-multiwellplates)forbiochemicalanalysisofGFPfusionproteinsand
theirinteractingpartners.
TheGFP-Trap specificallybindstomostcommonGFPderivates(seeFAQsforacompletelist).
NobindingtoredfluorescentproteinsderivedfromDsRedcanbedetected.
GFP-Trap featuresataglance:
RobustandversatiletoolforbiochemicalanalysesofGFP-fusionproteins
Shortincubationtimes(5 30min)
Quantitativeisolationoffusionproteinsandtransientlyboundfactorsfromcellextractsororganelles
Lowunspecificbinding
Nocontaminatingheavyandlightchainsofconventionalantibodies
ApplicableinChromatinImmunoprecipitation(ChIP)
UseGFP-Trap for:
Pulldowns/Immunoprecipitations
Co-IP
Massspectroscopy
Enzymeactivitymeasurements
ChIPanalysis
GFP-Trap_A
Description
GFP-Trap coupledtoagarosebeads
particlesize~80 m
Storagebuffer
20%EtOH
Storageinstructions
Shippedatambienttemperature.Uponreceiptstoreat4 stableforone
year.Donotfreeze.
Latest references
Castello, A. Fischer, B. Eichelbaum, K. Horos, R. Beckmann, B. Strein, C. Davey, N. Humphreys, D. Preiss, T. Steinmetz, L. Krijgsveld, J Hentze, M. (2012). Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins. Cell (PubMed) Ries, J. Kaplan, C. Platonova, E. Eghidi, H. Ewers, H. (2012). A simple, versatile method for GFP-base super-resolution microscopy via nanobodies. Nat. Methods. 10.1038. (PubMed) Pichler, G. Jack, A. Wolf, P. Hake, S. B. (2012). Versatile Toolbox for High Throughput Biochemical and Functional Studies with Fluorescent Fusion Proteins. PLoS One. 7: e36967. (PubMed) Gudesblat, G. E. Schneider-Pizon, J. Betti, C. Mayerhofer, J. Vanhoutte, I. van Dongen, W. Boeren, S. Zhiponova, M. de Vries, S. Jonak, C. Russinova, E. (2012). SPEECHLESS integrates brassinosteroid and stomata signalling pathways. Nat Cell Biol. (PubMed) Majumdar, A. Cesario, W. C. White-Grindley, E. Jiang, H. Ren, F. Khan, M. R. Li, L. Choi, E. M. Kannan, K. Guo, F. Unruh, J. Slaughter, B. Si, K. (2012). Critical role of amyloid-like oligomers of Drosophila Orb2 in the persistence of memory. Cell. 148: 515-29. (PubMed) Metzger, T. Gache, V. Xu, M. Cadot, B. Folker, E. S. Richardson, B. E. Gomes, E. R. Baylies, M. K. (2012). MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function. Nature. (PubMed) Wild, P. Farhan, H. McEwan, D. G. Wagner, S. Rogov, V. V. Brady, N. R. Richter, B. Korac, J. Waidmann, O. Choudhary, C. Dotsch, V. Bumann, D. Dikic, I. (2011). Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth. Science. 333: 228-33. (PubMed)北京乐博生物联系方式:
全国免费咨询热线:400-613-0504
电话:010-60606584/60606184
传真:010-58850899
邮箱:info@lab-bio.com
网站:www.lab-bio.com
地址:北京市海淀区清河三街95号同源大厦912室
江苏泰洁检测技术有限公司
江苏泰洁检测技术有限公司成立于2010年8月,注册资本1000万元人民币。座落于中国首批1
http://taijie.testrust.com/
美国信科检验认证集团深圳实验室
美国信科检验认证集团(SIEMIC)总部位于美国硅谷,在韩国,台湾,香港,深圳,南京,
http://wxz163.testrust.com/
中诺(虎门)质检仪器设备有限公司
http://sjl663040.testrust.com/

Description:
anti-p53VHHcoupledtoagarosebeadsforimmunoprecipitationofp53
Specificity:
Species-Reactivity:testedonhuman,mouse
Epitope:302-393aa
Applications:
Beadproperties:
Beadsize: ~90µm(cross-linked4%agarosebeads)
Storagebuffer:20%EtOH
Storageinstructions:
Shippedatambienttemperature.Uponreceiptstoreat4°C;stableforoneyear
2014年5月26日Chromotek的Nano-Trap®基于alpaca的单域抗体片段(VHHS) √小型 √极端稳定 √70℃仍保持稳定,functionalin2MNaClor0.5%SDS √高亲和力 √解离常数范围在sub-nM...