企业QQ
官方微信
Description Analyze and manipulate the intramolecular interaction between the N- and C-terminal domains of Androgen Receptor (AR) with ChromoTek Fluorescent-Two Hybrid (F2H®) AR. Wild-type (wt), as well as several mutant AR forms can be investigated.
Specificity AR is expressed as two separate domains Bait: AR ligand binding domain (AR-LBD) fused to GFP Prey: AR N-terminal domain (AR-NTD) fused to RFP Bait and prey interact.
Applications Detect early steps of Androgen Receptor activation Real-time agonists and antagonists screening Visualized in live mammalian cells
Available AR variants AR-LBD: wt, W741L, F876L, T877A, F876L-T877A
Click on the left picture for a live cell analysis example Live-cell F2H imaging of reversible interactions between AR-LBD-GFP (green) and AR-NTD-RFP (red) in F2H-BHK cells
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn