1.Myc-Trap?BackgroundTheMycTag,derivedfromthec-Mycprotein,isapopularepitopetagfordetectingtheexpressionofrecombinantproteinsinyeast,bacteria,insectandmammaliancellsystems.TheMyctagmaybefusedtoeithertheN-terminus,C-terminusofaproteinorinternal.TheshortlinearpeptidesequenceoftheMyc-tagisN-EQKLISEEDL-Candhasasizeofabout1.2kDa.
2.ChromoTekMyc-Trap?DescriptionChromoTekMyc-Trap?forImmunoprecipitation:TheChromoTekMyc-Trap?isasingledomainantibodyfragmentcoupledtoagarosebeadsforimmunoprecipitation/pulldownofMyc-taggedproteins.Myc-Trap?isavailableasbothagarose(productcodeyta-)andmagneticagarosebeads(ytma-)instandardpacksizesfor2(testsample),10,20,100,200,and400runseach.
3.PositioningStatementTheChromoTekMyc-Trap?isauniqueandpremiumtoolforimmunoprecipitationofMyc-taggedproteins.ItisdesignedtomeettherequirementsofsubsequentdownstreamapplicationsincludingmassspectrometryasitprovidespureextractsofMyc-taggedprotein.
4.ValuePropositionTheChromoTekMyc-Trap?isaconvenientandhigh-performingtool,readytouse,forfastandefficientone-stepimmunoprecipitationofMyc-taggedproteins.ItiscompatibletodownstreamapplicationsincludingmassspectrometricanalysisasitprovidespureextractsofMyc-taggedproteinwithoutcontaminatingheavyandlightchainpeptidesknownfromconventionalantibodies.
5.TargetedMarketsTheChromoTekMyc-Trap?targetedmarketscomprisebiochemistryandproteomicsresearchinacademiaandpharma/biotech.Duetolicensingreasonstheuseinproduction,fordiagnosticpurposes,therapeutics’developmentforhumanoranimalsubjects,andinclinicaltrialsisprohibited.
6.CustomersBiochemists,cellbiologist,molecularbiologists,biotechnologists.LikelythelaboratorieshavealreadyexperienceswithandpublicationsofMyc-taggedproteinsandtheirgeneticconstructs.ItislikelythatGFP/RFP/GST-Trap?customersmayusetheMyc-Trap?too.Thisisbecausemostcustomersutilizemultipleprotein/peptidetags.
7.Customers’ApplicationsTheChromoTekMyc-Trap?isdesignedforimmunoprecipitationofMyc-taggedproteinsandtheirinteractingfactorsfromcellororganelleextractsandlysatesforamultitudeofbiochemicalanalysessuchasImmunoprecipitation(IP)/co-IP,massspectrometry,enzymeactivitymeasurementsandChIP/RIPanalysis.
8.CompetitionCompetitorsincludeSigma,EMD,CellSignalling,Roche,Abcam,Pierce,Clontech,andBiomol(Bethyl),whichareallusingclassicalantibodiescoupledtoagarose.
9.SalesStrategyWeadvisetohavecustomerstestingtheMyc-Trap?.ThereforeweofferMyc-Trap?testsamples.Packsizeissufficientfor2IPruns.
10.KeyWordsMyc,Myc-Tag,c-Myc,c-Myc-Tag,Immunoprecipitation,Pull-Down,anti-Myc-Tagantibody,Mycantibody,anti-Mycantibody,Myc-peptide,Masspectrometry/Spectrometer
