FormuMax/Trans-Hi™ - In vitro Transfection Reagent/1.0 mL/F90101TH-10

Trans-Hi™ is a superior transfection reagent capable to compete against the leading products on the market. It is based on a proprietary polymer technology. It forms stable nanoparticles with DNA and rapidly releases the DNA molecules upon endocytosis into the cytoplasm. It is a broad spectrum transfection regent offering superior and reproducible transfection efficiency on the common cells such as Hela, CHO, HEK293 and many hard-to-transfect mammalian cells with minimal toxicity. Trans-Hi™is very cost-efficient and 1.0mL is sufficient for over 660 transfections in 24 well plates.

Key Features for Trans-Hi^TM

• Excellent transfection for single DNA transfection or multi DNA co-transfection
• Rapid onset and long-lasting transfection
• Suitable for very long DNAs
• Simple and easy to use transfection procedure
• Robust and reproducible transfection
• High level of recombinant protein production
• Very cost efficient
• Long shelf-life: 12 months

Specifications:

Protocols

Trans-Hi™General Protocol For In Vitro Transfection in Mammalian Cells

Trans-Hi™ - Protocol for Transfecting 293 and CHO Suspension Cells

Trans-Hi™ - Protocol for Generation of rAAV

Trans-Hi™-Technical Tips For Good In Vitro Transfection

Comparison study ofIn vitro plasmid DNA transfection of Trans-Hi^TM and Lipofectamine®2000in HEK293T cells with GFP vector using the manufacture recommended reagent/DNA ratio of 3:1 (µL/µg) and 0.5µg DNA per wellused for both products in 24 well plate 30 hours post transfection.

Trans-Hi™/DNA ratio (µL/µg) (HEK293T)

Study of the transfection efficiency as a function of transfection reagent/DNA ratio indicating optimal tranfection efficiency achieved at Trans-Hi™/DNA ratio of 3:1 (µL/µg) (HEK293T cells in24 well plate 30 hours post transfection, 0.5ug DNA per well).

Head-to-head comparisonTrans-Hi™ against Lipofectamine 2000HEK293A cells with GFP vector (reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well), 24 well plate 48 hours post transfection).

Comparison study ofIn vitroplasmid DNA transfection of Trans-Hi™and Lipofectamine®2000in Hela cellswith GFP vector ((reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well),24 well plate 48 hours post transfection).

Broad Transfection Spectrum for Mammalian Cell Types